紫外辐射灭活大黄鱼和黄姑鱼精子及其激活大黄鱼卵子发育为胚胎的效果  

Effects of UV irradiation on motility of sperm in Larimichthys crocea and Nibea albiflora and development of fertilized oocytes in L. crocea

作　　者：汪倩凤 沈伟良 刘成 王迪[1] 侯聪聪 竺俊全[1,3] 吴雄飞 WANG Qianfeng;SHEN Weiliang;LIU Cheng;WANG Di;HOU Congcong;ZHU Junquan;WU Xiongfei(College of Marine Sciences, Ningbo University, Ningbo 315211, China;Marine and Fisheries Research Institute, Ningbo 315012, China;Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315211,China)

机构地区：[1]宁波大学教育部应用海洋生物技术重点实验室,浙江宁波315211 [2]宁波市海洋与渔业研究院,浙江宁波315012 [3]浙江省海洋高效健康养殖协同创新中心,浙江宁波315211

出　　处：《中国水产科学》2017年第6期1203-1212,共10页Journal of Fishery Sciences of China

基　　金：宁波市科技计划重大项目(2015C110005);浙江省科技计划重大项目(2016C02055-7)

摘　　要：为探讨大黄鱼(Larimichthys crocea)和黄姑鱼(Nibea albiflora)精子的紫外辐射灭活适宜剂量及其激活大黄鱼卵子发育为胚胎的效果,以Ringer氏液为稀释液,按1:30稀释大黄鱼和黄姑鱼精子,采用自制紫外灭活装置[紫外辐射强度2200μW/(cm^2·s),紫外波长254 nm]对这2种鱼的精子进行紫外照射处理及活力测定,然后与正常大黄鱼卵子进行人工授精,授精后一部分卵未作冷休克处理,另一部分卵进行了冷休克处理(受精2 min 30 s,3℃海水,冷休克10 min),并进行了早期胚胎成活率和仔鱼孵化率的测定与比较。结果显示:1)大黄鱼和黄姑鱼精子的激活率与紫外照射处理时间呈负相关,精子的快速运动时间变化呈典型的Hertwig效应。2)未冷休克组中大黄鱼和黄姑鱼诱导的早期胚胎成活率与精子的紫外照射时间总体呈负相关,而仔鱼孵化率呈Hertwig效应。3)冷休克组中大黄鱼和黄姑鱼精子诱导的早期胚胎成活率和仔鱼孵化率随紫外照射时间的增加呈Hertwig效应,分别于2 min 20 s和1min 30 s时达相对峰值,此时,大黄鱼早期胚胎成活率和仔鱼孵化率分别为(38.3±4.3)%和(66.5±5.1)%,黄姑鱼早期胚胎成活率和仔鱼孵化率分别为(43.3±3.3)%和(67.7±6.3)%。分析认为,大黄鱼和黄姑鱼精子遗传失活的紫外辐射剂量分别以308 m J/cm^2和198 m J/cm^2为佳。本研究旨在为大黄鱼雌核发育技术的改进提供依据。This study investigated the effects of UV irradiation on sperm motility and duration of sperm activity and determined the optimal UV irradiation dosage to genetically inactivate Larimichthys crocea and Nibea albiflora sperm,while retaining adequate motility to fertilize L.crocea eggs.Milt from several males was diluted1:30with Ringer's solution and UV irradiated at an intensity of2200μW/(cm2·s),and used to fertilize L.crocea eggs.The fertilized eggs were divided into a cold-shock group(3℃for10min at2min30s after fertilization)and a control group without cold-shock.Early embryo survival rate and larvae hatching rate were measured and compared.The results showed that:1)motility of L.crocea and N.albiflora sperm was negatively correlated with UV treatment time,and the rapid changes in sperm showed a typical Hertwig effect;2)early embryo survival rate induced by L.crocea and N.albiflora sperm in the control groups was negatively correlated with the UV exposure time,while the larvae hatching rate showed a typical Hertwig effect;3)early embryo survival rate and larvae hatching rate induced by L.crocea and N.albiflora sperm in the cold-shock group showed a typical Hertwig effect with the increase in UV irradiation time,reaching peaks at2min20s and1min30s,respectively(the peak values of the survival and hatching rates of L.crocea were(38.3±4.3)%and(66.5±5.1)%,respectively;the peak values of the survival and hatching rates of N.albiflora were(43.3±3.3)%and(67.7±6.3)%,respectively).The results of this study indicated that the optimal UV dosages for L.crocea and N.albiflora sperm to activate L.crocea eggs were308mJ/cm2and198mJ/cm2,respectively.These results provide information for the improvement of gynogenetic technology for L.crocea.

关 键 词：大黄鱼 黄姑鱼 紫外照射 精子活力 胚胎成活率 孵化率 

分 类 号：Q96[生物学—昆虫学]