SSR Primer Screening for Marchantia convoluta by SAM Method  

作　　者：Yuefeng WANG Fengfeng XIE Yuqing ZHOU Peilin DU Hua ZHU Jianbei TENG 

机构地区：[1]Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [2]Guangxi University of Chinese Medicine,Nanning 530200,China [3]First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China

出　　处：《Agricultural Biotechnology》2017年第5期1-5,10,共6页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of China(No.81260620);Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20);Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32);Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16);Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars

摘　　要：[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.

关 键 词：MARCHANTIA convoluta DNA extraction SAM method SSR PRIMER SCREENING 

分 类 号：Q943.2[生物学—植物学]