PRIMER

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Genome-wide primer scan (GPS): a python package for a flexible, reliable and large-scale primer design toolkit
《Frontiers of Computer Science》2025年第2期127-128,共2页Wencong HE Yan ZHUO Chen WANG Yemei HUANG Xuelei ZANG Chen YANG Hengyu DENG Yangyu ZHOU Jing LIU Ping ZHANG Xinying XUE Liye ZHANG 
supported by the National Key R&D Program of China(SQ2021YFC2300067).
1 Introduction Microbial infections represent significant global health challenges with far-reaching implications.In contemporary medical practice,quantitative polymerase chain reaction(qPCR)stands as an important tec...
关键词：PYTHON false typically 
Imaging evaluation of periprosthetic loosening:A primer for the general radiologist
《World Journal of Radiology》2025年第2期1-10,共10页Sahil S Shet Eid Kakish Stephen Christopher Murphy Ryan Roopnarinesingh Stephen P Power Michael M Maher David J Ryan 
In response to an ageing global population,the primary hip and knee arthroplasty rate continues to increase.Although an effective treatment,up to 25%patients may require revision arthroplasty during their lifetime,com...
关键词：Periprosthetic loosening Total hip replacement Total knee replacement Diagnostic radiology Aseptic loosening 
IRS-enabled NOMA communication systems:A network architecture primer with future trends and challenges
《Digital Communications and Networks》2024年第5期1503-1528,共26页Haleema Sadia Ahmad Kamal Hassan Ziaul Haq Abbas Ghulam Abbas Muhammad Waqas Zhu Han 
supported by Higher Education Commission(HEC)of Pakistan through its National Research Program for Universities(NRPU)[Ref.No.20-14560/NRPU/R&D/HEC/2021];support provided by HEC。
Non-Orthogonal Multiple Access(NOMA)has already proven to be an effective multiple access scheme for5th Generation(5G)wireless networks.It provides improved performance in terms of system throughput,spectral efficienc...
关键词：Intelligent reflecting surface Non-orthogonal multiple access 6G BEAMFORMING Sum rate Energy efficiency 
Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced(TAIL)-PCR
《Plant Communications》2024年第9期25-37,共13页Sheng Zhao Yue Wang Zhenghang Zhu Peng Chen Wuge Liu Chongrong Wang Hong Lu Yong Xiang Yuwen Liu Qian Qian Yuxiao Chang 
supported by the Science and Technology Planning Project of Guangdong Province(2022B0202060002);the National Natural Science Foundation of China(32300340,32172086);the R&D program of Shenzhen(KCXFZ20211020164207012);the R&D Program in Key Areas of Guangdong Province(2021B0707010006);ACKNOWLEDGMENTS We thank Lili Dong(China National Center for Bioinformation/Beijing Institute of Genomics,Chinese Academy of Sciences)for assistance with uploading the raw sequencing data.Y.C.,S.Z.,P.C.,et al.are listed as co-inventors on a patent application(CN202211418)。
Whole-genome genotyping(WGG)stands as a pivotal element in genomic-assisted plant breeding.Nevertheless,sequencing-based approaches for WGG continue to be costly,primarily owing to the high expenses associated with li...
关键词：whole-genome genotyping primer-template mismatched annealing specific primer arbitrary degenerate primer molecular breeding 
不同功能成分底涂剂对氧化锆耐久粘接强度的影响
《口腔疾病防治》2024年第7期502-508,共7页赵莉 赵克 
国家自然科学基金面上项目(82071156)。
目的比较含10-甲基丙烯酰氧癸基磷酸酯(10-metracryloyloxdecyl dyhidrogen phosphate,10-MDP)和硅烷的混合型底涂剂与MDP单一功能成分型底涂剂对氧化锆初期及耐久粘接强度的影响。方法制作氧化锆标准试件84个,随机分成4组(n=21),分别...
关键词：氧化锆 陶瓷 粘接 底涂剂 Z‐Prime plus Clearfil Ceramic Primer 冷热循环老化 
SubEpiPredict:A tutorial-based primer and toolbox for fitting and forecasting growth trajectories using the ensemble n-sub-epidemic modeling framework
《Infectious Disease Modelling》2024年第2期411-436,共26页Gerardo Chowell Sushma Dahal Amanda Bleichrodt Amna Tariq James M.Hyman Ruiyan Luo 
G.C.is partially supported from NSF grants 2125246 and 2026797 and R01 GM 130900.
An ensemble n-sub-epidemic modeling framework that integrates sub-epidemics to capture complex temporal dynamics has demonstrated powerful forecasting capability in previous works.This modeling framework can character...
关键词：n-Sub-epidemic model Forecasting MATLAB Sub-epidemics Phenomenological models Performance metrics Model evaluation 
A single degenerated primer significantly improves COX1 barcoding performance in soil nematode community profiling
《Soil Ecology Letters》2024年第2期31-44,共14页Yincai Ren Dorota L. Porazinska Quanhui Ma Shuhan Liu Hongmei Li Xue Qing 
supported by the National Natural Science Foundation of China(Grant number 32001876).
A new COX1 primer for soil nematode metabarcoding was designed,and this primer outperforms other commonly used COX1 primer pairs in species recovery and quantity of PCR products.•The lack of reference database is the ...
关键词：degenerated primers DNA metabarcoding mitochondrial cytochrome oxidase c subunit I gene PHYLOGENY ribosomal RNA gene soil nematodes 
Direct-to-indirect mapping for optimal low-thrust trajectories
《Astrodynamics》2024年第1期27-46,共20页David Ottesen Ryan P.Russell 
Optimal,many-revolution spacecraft trajectories are challenging to solve.A connection is made for a class of models between optimal direct and indirect solutions.For transfers that minimize thrust-acceleration-squared...
关键词：direct optimization indirect optimization impulsive maneuvers Lambert's problem two-body dynamics primer vectortheory 
Optimal impulsive rendezvous for highly elliptical orbits using linear primer vector theory被引量：1
《Chinese Journal of Aeronautics》2024年第3期194-207,共14页Maozhang ZHENG Jianjun LUO Zhaohui DANG 
supported by National Natural Science Foundation of China(No.12172288);National Key Basic Research Program of China:Gravitational Wave Detection Project(Nos.2021YFC2202601 and 2021YFC2202603);General Program of Natural Science Foundation of Higher Education of Jiangsu Province(No.21KJB590001)。
In this paper,minimum-fuel rendezvous is investigated for the case in which the reference orbit is highly elliptic.To this end,the well-known Tschauner-Hempel equations are used to describe the relative motions betwee...
关键词：Orbital transfer Optimal trajectory Primer vector Tschauner-Hempel equations Globally planning Fast planning 
LSQP‑DB:a species‑specific quantitative PCR primer database for 307 Lactobacillaceae species
《Systems Microbiology and Biomanufacturing》2023年第4期593-601,共9页Rubing Du Shilei Wang Qun Wu Yan Xu 
supported by the National Natural Science Foundation of China(32172175);National First-Class Discipline Program of Light Industry Technology and Engineering(LITE2018-12);the Priority Academic Program Development of Jiangsu Higher Education Institutions,the 111 Project(No.111-2-06);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21-2029).
Quantitative real-time PCR is widely used to determine absolute abundance of microbes in food fermentation.However,it remains challenges in the application for quantification at the species level due to the difficulty...
关键词：Lactobacillaceae Primer database Species-specific primer qPCR 
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