SRPX2在胃癌中的表达及对细胞侵袭的影响  

Expression of SRPX2 in human gastric carcinoma and its role in cell invasion

作　　者：李婷[1] 彭波[1] Ting Li;Bo Peng(Department of Gastroenterology, Tongren Hospital of Wuhan University(the Third Wuhan Hospital), Wuhan, Hubei 430070, China)

机构地区：[1]武汉大学附属同仁医院(武汉市第三医院)消化内科,湖北武汉430070

出　　处：《中国现代医学杂志》2017年第28期36-40,共5页China Journal of Modern Medicine

摘　　要：目的探讨含sushi重复蛋白X连锁2(SRPX2)在人胃癌(GC)中的表达及对胃癌MGC-803细胞侵袭能力的影响。方法收集2014年3月1日-2015年3月1日于该院消化内科行手术切除的45例胃癌及对应癌旁组织。检测SRPX2 mRNA及蛋白在胃癌及癌旁组织中的表达情况,分析胃癌组织中SRPX2蛋白表达对肿瘤临床表型的影响。通过si RNA下调胃癌MGC-803细胞中SRPX2的表达,通过Transwell侵袭小室检测沉默SRPX2之后MGC-803细胞侵袭能力的变化;同时检测沉默SRPX2后MGC-803细胞内基质金属蛋白酶2(MMP-2)表达水平的改变。结果 SRPX2在胃癌组织中表达升高,胃癌组织高表达SRPX2与肿瘤淋巴结转移及高TNM分期(Ⅲ+Ⅳ期)相关(P<0.05);沉默SRPX2表达可抑制胃癌MGC-803细胞的侵袭能力并同时下调了MGC-803细胞中MMP-2的表达。结论胃癌组织中高表达的SRPX2与肿瘤转移表型关系密切,沉默SRPX2可能通过下调MMP-2的表达而抑制胃癌细胞侵袭。Objective To detect the expression of Sushi Repeat Containing Protein X Linked2(SRPX2)inhuman gastric carcinoma(GC)and its role in cell invasion.Methods qRT-PCR and immunohistochemistry wereapplied to detect the expression of SRPX2in GC(n=45)and matched tumor-adjacent tissues.The correlationsbetween SRPX2expression and clinical features were analyzed by Pearson chi-square test.siRNA was used tosilence the expression of SRPX2in MGC-803cells.The effect of SRPX2on cell invasion was measured by transwellassay.MMP-2expression was detected after silencing SRPX2expression.Results The expression of SRPX2wassignificantly up-regulated in the GC tissues,and the high expression of SRPX2was associated with the lymphaticmetastasis and advanced TNM stages(stageⅢandⅣ)(P<0.05).Silencing SRPX2could inhibit cell invasionand MMP-2expression in the MGC-803cells.Conclusions The expression of SRPX2is significantly higher inGC tissues,which is correlated to tumor metastases.Silencing SRPX2could achieve an anti-cancer role throughinhibition of cell invasion.

关 键 词：含sushi重复蛋白X连锁2 胃癌 表达 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]