不同抗生素和防腐剂对小球藻Chlorella sorokiniana细胞生长的影响  

Effects of different antibiotics and preservatives on Chlorella sorokiniana cell growth

作　　者：李亚军[1] 朱为菊[1] 费小雯[2] 邓晓东[1] LI Ya-jun;ZHU Wei-ju;FEI Xiao-wen;DENG Xiao-dong(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Torpical Crops,Ministry of Agriculture,Haikou 571101,China;Department of Biochemistry,Hainan Medical College,Haikou 571199,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口571101 [2]海南医学院理学院,海口571199

出　　处：《南方农业学报》2017年第11期2078-2085,共8页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31360051);海南省重点研发计划项目(ZDYF2016021);中央级公益性科研院所基本科研业务费专项项目(1630052016009)

摘　　要：【目的】分析不同抗生素和防腐剂对小球藻Chlorella sorokiniana细胞生长的影响,筛选出适宜的抗生素和防腐剂种类及其剂量,为建立小球藻C.sorokiniana无菌培养体系提供参考依据。【方法】在HSM培养基中分别添加10、20、40、60、80和100μg/mL抗生素,包括氯霉素、链霉素、青霉素、庆大霉素和头孢噻肟,以及山梨酸钾(50、100、150和200μg/mL)、乳酸钠(0.5%、1.0%和2.0%)、富马酸二甲酯(0.1、0.5、1.0、5.0、10.0、25.0和50.0μg/mL)等防腐剂,培养3 d后测定小球藻C.sorokiniana的细胞密度、叶绿素含量及光系统Ⅱ最大光化学量子产量(Fv/F_m)。【结果】添加10~100μg/mL青霉素或10~80μg/mL头孢噻肟对小球藻C.sorokiniana细胞密度、叶绿素含量和Fv/F_m的影响均不显著(P>0.05,下同);添加100μg/mL头孢噻肟会抑制藻细胞生长,抑制率为18.89%,并引起叶绿素含量和Fv/F_m极显著下降(P<0.01,下同)。氯霉素、链霉素和庆大霉素等蛋白质合成抑制类抗生素对小球藻C.sorokiniana细胞的毒性较强,高于10μg/mL的链霉素和庆大霉素及高于20μg/mL的氯霉素均极显著抑制藻细胞生长,对应的叶绿素含量和Fv/F_m也极显著下降。3种防腐剂中,山梨酸钾对小球藻C.sorokiniana细胞生长无显著影响,添加200μg/mL山梨酸钾还会促进细胞叶绿素含量增加;乳酸钠对小球藻C.sorokiniana细胞有一定的毒性作用,其藻细胞密度、叶绿素含量和Fv/F_m随添加浓度增加而降低;添加0.1~10.0μg/mL富马酸二甲酯不影响小球藻C.sorokiniana的细胞密度及叶绿素含量,但Fv/F_m极显著下降。【结论】建立小球藻C.sorokiniana无菌培养体系时宜选用青霉素和头孢噻肟为抗生素、山梨酸钾为防腐剂,其推荐使用浓度分别为青霉素100μg/mL、头孢噻肟80μg/mL、山梨酸钾200μg/mL。【Objective】Effects of different antibiotics and preservatives on growth of Chlorella sorokiniana cells were analyzed,and optimal antibiotics and preservatives and doses were screened to provide reference for establishing axenic culture of C.sorokiniana.【Method】Antibiotics and preservatives were added in HSM media including chloramphenicol,streptomycin,penicillin,gentamicin and cefotaxime sodium(10,20,40,60,80and100μg/mL),potassium sorbate(50,100,150and200μg/mL),lactic acid bacteria(0.5%,1.0%and2.0%),dimethyl fumarate(0.1,0.5,1.0,5.0,10.0,25.0and50.0μg/mL).Cell density,chlorophyll content and photosystemⅡmaximal photochemical efficiency(Fv/Fm)were determinated after3d cultivation.【Result】Cell density,chlorophyll content and Fv/Fm of C.sorokiniana were not significantly affected by10-100μg/mL penicillin and10-80μg/mL cefotaxime sodium(P>0.05,the same below).Adding100μg/mL cefotaxime sodium inhibited cell growth with inhibition rate of18.89%,and also decreased chlorophyll content and Fv/Fm extremely significantly(P<0.01,the same below).Compared with control,protein synthesis inhibitory antibiotics like chloramphenicol,streptomycin and gentamicin were more toxic to C.sorokiniana.Streptomycin and gentamicin with concentrations over10μg/mL and chloramphenicol with concentrations over20μg/mL had extremely significant inhibition effects on cell growth,and the corresponding chlorophyll content and Fv/Fm dropped extremely significantly.Among the three preservatives,potassium sorbate did not affect C.sorokiniana cell growth significantly.Furthermore,the chlorophyll content was enhanced by200μg/mL potassium sorbate.Lactic acid bacteria was toxic to C.sorokiniana cell at certain extent,cell density,chlorophyll content and Fv/Fm of C.sorokiniana decreased as the concentration increased.Adding0.1-10.0μg/mL dimethyl fumarate did not affect the cell density and chlorophyll content of C.sorokiniana,but the Fv/Fm significantly decreased.【Conclusion】In establishing axenic culture of C.sorokiniana,the suit

关 键 词：小球藻Chlorella sorokiniana 抗生素 防腐剂 细胞密度 叶绿素含量 FV/FM 

分 类 号：S963.213[农业科学—水产养殖]