溶瘤腺病毒CD55-TRAIL-IETD-MnSOD联合阿霉素抑制肝癌细胞HepG2生长  

Synergistic Suppression Effect on Growth of Hepatocellular Carcinoma Cells HepG2 by Combining Oncolytic Adenovirus CD55-TRAIL-IETD-MnSOD with Doxorubicin Hydrochloride

作　　者：肖伯端 秦云 应畅[1] 王彬蓉 龙菲 王毅刚[1] XIAO Bo-Duan;QIN Yun;YING Chang;WANG Bin-Rong;LONG Fei;WANG Yi-Gang(College of Life Science, Zhejiang Sci-Tech University, Hangzhou 230009, China;Xinyuan Institute of Medicine and Biotechnology,Hangzhou 230009, China)

机构地区：[1]浙江理工大学生命科学学院,新元医学与生物技术研究所,浙江杭州310018

出　　处：《生物技术通讯》2017年第6期725-730,共6页Letters in Biotechnology

摘　　要：目的:研究溶瘤腺病毒CD55-TRAIL-IETD-MnSOD与阿霉素联合使用对肝癌细胞HepG2生长的影响。方法:MTT法检测经CD55-TRAIL-IETD-MnSOD与阿霉素联合处理后HepG2细胞生存率的变化,Hoechst33342染色及流式细胞术检测联合处理诱导细胞凋亡时细胞形态变化及凋亡细胞数量,Western印迹检测联合处理所引发的凋亡通路。结果:MTT实验显示,联合使用CD55-TRAIL-IETD-MnSOD和阿霉素能更有效地抑制HepG2细胞的生长,且阿霉素与CD55-TRAIL-IETD-MnSOD是协同作用;Hochest染色实验和流式细胞术实验结果均显示联合使用CD55-TRAIL-IETD-MnSOD和阿霉素增强了诱导细胞凋亡的作用;Western印迹表明CD55-TRAIL-IETD-MnSOD和阿霉素联合使用激活了Caspase细胞凋亡途径。结论:阿霉素能促进溶瘤腺病毒CD55-TRAIL-IETD-MnSOD的复制,联合阿霉素和溶瘤腺病毒CD55-TRAIL-IETD-MnSOD可更有效激活Caspase细胞凋亡通路,抑制肝癌细胞HepG2生长。Objective:To study the effect of oncolytic adenovirus CD55-TRAIL-IETD-MnSOD combined withdoxorubicin hydrochloride(DOX)on the growth of hepatocellular carcinoma cells HepG2.Methods:MTT methodwas used to detect HepG2survival after treatment CD55-TRAIL-IETD-MnSOD combined with DOX.Cell morpho?logical changes were detected by Hoechst33342staining and flow cytometry technology to detect the number ofapoptotic cells.Western blotting was used to detect apoptotic pathways triggered by combined treatment.Results:MTT experiment showed that through combination of CD55-TRAIL-IETD-MnSOD and DOX can inhibit the growthof HepG2cells in a synergic way and more effectively.Hochest dyeing experiments and flow cytometry technologyresults showed CD55-TRAIL-IETD-MnSOD and DOX enhance the induction of cell apoptosis.Western blot verified CD55-TRAIL-IETD-MnSOD combined with DOX activated Caspase apoptosis pathway.Conclusion:DOX canpromote the replication of CD55-TRAIL-IETD-MnSOD,therefore,the apoptosis pathway of Caspase can be effectively activated,and the HepG2growth can be inhibited.

关 键 词：溶瘤腺病毒 CD55-TRAIL-IETD-MnSOD 阿霉素 肝癌细胞HEPG2 

分 类 号：Q24[生物学—细胞生物学] R730[医药卫生—肿瘤]