siRNA干涉PIM-3表达对胶质瘤细胞增殖和糖代谢的影响  

Effects of PIM-3 siRNA on Glioma Cell Proliferation and Glucose Metabolism

作　　者：权俊杰[1] 屈建强[1] 周乐[1] 张熙[1] 侯娟茹 QUAN Jun-Jie;QU Jian-Qiang;ZHOU Le;ZHANG Xi;HOU Juan-Ru(Department of Neurosurgery, Second Affiliated Hospital, Xi'an 710004,Medical School of Xi'an Jiaotong University, China;Operation Department, First Affiliated Hospital, Xi'an 710061, Medical School of Xi'an Jiaotong University, China)

机构地区：[1]西安交通大学医学院第二附属医院神经外科,西安710004 [2]西安交通大学医学院第一附属医院手术部,西安710061

出　　处：《生物技术通讯》2017年第6期773-776,共4页Letters in Biotechnology

基　　金：陕西省自然科学基金(2016JM8098)

摘　　要：目的:利用小干扰RNA(siRNA)在胶质瘤细胞干涉PIM-3的表达,观察细胞增殖能力和代谢的变化,并探讨相关机制。方法:转染PIM-3 siRNA入胶质瘤细胞U87-MG和U251,利用MTT实验和平板克隆实验检测细胞增殖能力的变化,利用流式细胞仪测定细胞糖摄取能力,并通过Western印迹检测葡萄糖转运蛋白GLUT1的表达变化。结果:利用siRNA干涉了PIM-3在胶质瘤细胞中的表达;PIM-3表达降低后,细胞增殖能力下降,葡萄糖摄取能力减弱,GLUT1蛋白表达降低。结论:PIM-3对胶质瘤细胞的增殖和代谢重编程具有调控作用,并主要通过影响细胞的糖代谢来实现。Objective:To observe cell proliferation and metabolism alterations,and discuss the underlying mechanism by inhibition of PIM-3expression with small interference RNA(siRNA)in glioma cells.Methods:Transfect U87-MG and U251cells with PIM-3siRNA.Detect cell proliferation ability with MTT and colony formation assays.Detect glucose uptake capacity with flow cytometry.Results:PIM-3expression was repressed in glioma cells with PIM-3siRNA.Cell proliferation and glucose uptake abilities were decreased as PIM-3was repressed.PIM-3inhibition decreased GLUT1expression.Conclusion:PIM-3plays a key role in regulation of glioma cell proliferation and metabolic reprogramming predominantly through the regulation of glucose metabolism.

关 键 词：PIM-3 胶质瘤 糖代谢 小干扰RNA 

分 类 号：R739.4[医药卫生—肿瘤] Q78[医药卫生—临床医学]