Isolation of three cyanins from Lonicera caerulea L. fruits and its anticancer activity  

作　　者：LUO Zhou-fei YANG Yuan WANG Liang-sheng LI Hai-pu 罗洲飞;杨远;王亮生;李海普

机构地区：[1]Center for Environment and Water Resources,College of Chemistry and Chemical Engineering,Central South University [2]Key Laboratory of Plant Resources,Beijing Botanical Garden,Institute of Botany,Chinese Academy of Sciences

出　　处：《Journal of Central South University》2017年第7期1573-1581,共9页中南大学学报（英文版）

基　　金：Project(KSCX2-YW-N-043)supported by the Knowledge Innovation Program of the Chinese Academy of Sciences,China

摘　　要：Lonicera caerulea L.fruit is an excellent source of bioactive compounds.An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients.High-speed counter-current chromatography(HSCCC) was applied to isolate cyanins from Lonicera caerulea fruits with a biphasic solvent system composed of methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid(2:2:1:5:0.01,volume ratio).1.41 mg of cyanidin 3,5-O-diglucoside,1.08 mg of cyanidin 3-O-rutinoside and 6.38 mg of cyanidin 3-O-glucoside were obtained from 40 mg of crude extract.The purities of these compounds were 95.8%,92.4% and 97.6%,respectively,as identified by high-performance liquid chromatography–diode array detection(HPLC-DAD) and high-performance liquid chromatography-electrospray ionization mass spectrometryn(HPLC-ESI/MSn).In addition,the dominant anthocyanin,cyanidin 3-O-glucoside was demonstrated cytotoxic response of human hepatocarcinoma SMMC-7721 cells,inducing live cancer cell apoptotic by flow cytometric analysis.Lonicera caerulea L. fruit is an excellent source of bioactive compounds. An efficient separation method of cyanins is important for the development of many value-added products and functional food ingredients. High-speed counter-current chromatography (HSCCC) was applied to isolate cyanins from Lonicera caerulea fruits with a biphasic solvent system composed of methyl tert-butyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (2:2:1:5:0.01, volume ratio). 1.41 mg of cyanidin 3, 5-O-diglucoside, 1.08 mg of cyanidin 3-O-rutinoside and 6.38 mg of cyanidin 3-O-glucoside were obtained from 40 mg of crude extract. The purities of these compounds were 95.8%, 92.4% and 97.6%, respectively, as identified by high-performance liquid chromatography-diode array detection (HPLC-DAD) and high-performance liquid chromatography-electrospray ionization mass spectrometry(n) (HPLC-ESI/MSn). In addition, the dominant anthocyanin, cyanidin 3-O-glucoside was demonstrated cytotoxic response of human hepatocarcinoma SMMC-7721 cells, inducing live cancer cell apoptotic by flow cytometric analysis.

关 键 词：cyanins CYANIDIN 3-O-glucoside ISOLATION ANTICANCER HSCCC HPLC-ESI-MSn 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]