PrepFiler Express BTA^(TM)裂解液联合硅珠法快速提取骨骼DNA  

PrepFiler Express BTA^(TM) Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

作　　者：丁少成[1] 张怀才[1] 高林林[1] DING Shao-cheng;ZHANG Huai-cai;GAO Lin-lin(Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310016, China)

机构地区：[1]杭州市公安局刑事科学技术研究所,浙江杭州310016

出　　处：《法医学杂志》2017年第5期514-515,521,共3页Journal of Forensic Medicine

摘　　要：目的建立方便、快捷的提取骨骼DNA的方法。方法对15份长骨样本清洗消毒后,采用电钻钻取骨屑,使用PrepFiler Express BTA^(TM)裂解液进行裂解消化后,应用手工硅珠吸附纯化进行DNA提取检验。结果有14份样本获得满意的STR分型,仅1份样本未获得STR分型,检出率为93.33%。结论本研究建立的骨骼DNA快速提取方法操作简单、便捷、有效,适应性强,值得推广应用。Objective To establish a convenient and rapid method for extracting DNA from bone.Methods Fifteen long bone samples were washed and sterilized.The skeletal fragments were obtained by electric drill,and lysed by PrepFiler Express BTATM lysis buffer.DNA was then manually extracted by silicon microbeads for further analysis.Results STR genotyping was successfully obtained in14out of the15samples,and the detection rate was93.33%.Conclusion The method for DNA extraction from bone established in present study is convenient,quick,effective,and with a strong applicability,which is worth spreading and applying.

关 键 词：法医遗传学 骨骼 DNA PrepFiler EXPRESS BTATM 硅珠法 

分 类 号：DF795.2[医药卫生—法医学]