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作品数:2517被引量:1242H指数:14
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小鼠胚胎脊髓神经干细胞提取与传代培养:3种常用消化酶优劣的比较与分析
《中国组织工程研究》2025年第31期6609-6615,共7页罗丹 葛志林 侯永辉 王万舜 詹吉恒 侯宇 林定坤 陈树东 
国家自然科学基金项目(81704095),项目负责人:陈树东;广州市基础研究计划基础与应用基础研究项目(202102020542),项目负责人:陈树东;国家自然科学基金项目(82074451),项目负责人:林定坤。
背景:在神经干细胞的研究及应用过程中,细胞的培养与传代是关键环节,直接影响到细胞的质量和实验结果。寻找一种最适宜的消化酶类,对维持胚胎小鼠脊髓神经干细胞的生物学特性并增强其传代效率,具有重要意义。目的:探讨胚胎小鼠脊髓神经...
关键词:神经干细胞 C57BL/6小鼠 胚胎 脊髓 胰蛋白酶 木瓜蛋白酶 TrypLE^(TM)Express酶 工程化干细胞 
International Transport Corridors and the Growth of China's Environmental Goods Exports:Evidence from the China-Europe Railway Express
《China & World Economy》2025年第2期98-137,共40页Chang Song Yibing Ding Yingjie Fu Jingyi Liu 
supported by the Youth Projects of the National Social Science Foundation of China(No.23CJL014).
This paper investigates the mechanisms and effects of international transport corridors on the growth of China's environmental goods exports.Using bilateral data between Chinese provinces and their trading partners,th...
关键词:Belt and Road Initiative China-Europe Railway Express export of environmenta goods green trade international transport corridor 
Application of intensive construction technology in the grand Paris express project:A review
《Frontiers of Structural and Civil Engineering》2025年第3期488-501,共14页Yi ZHANG 
With 200 km of new lines and 68 new stations,the Grand Paris Express(GPE)project is currently the biggest transport project under construction in Europe.Starting in 2010,the GPE project involves an ambitious schedule ...
关键词:megaproject underground works construction method innovative technology environment 
News Express
《China Forex》2024年第4期11-11,共1页
To implement the decisions and arrangements of the third plenary session of the 20th CPC Central Committee on"establishing a long-term mechanism to effectively enhance the stability in the capital market",to further s...
关键词:COMPANIES arrangement establishing 
Does the China Railway Express Promote Equitable Regional Development?Evidence from Manufacturing Relocation
《China & World Economy》2024年第6期128-159,共32页An Pan Wenna Zhang Yige Du Xunpeng Shi 
support from the National Social Science Fund of China (No.22BGJ049);the National Natural Science Foundation of China (No.72174056).
The China Railway Express(CRE)has improved regional economic integration with external markets and has reshaped regional development by encouraging the relocation of manufacturing activities("manufacturing transfer")....
关键词:China Railway Express equitable regional development infrastructure construction manufacturing transfer 
Comparative study on the expression characteristics of transgenes inserted into the Gt(ROSA)26Sor and H11 loci in mice
《Acta Biochimica et Biophysica Sinica》2024年第11期1687-1698,共12页Zhilan Yu Jiahao Shi Jingyu Zhang Youbing Wu Ruling Shen Jian Fei 
This work was supported by the grants from the National Key R&D Program of China(No.2019YFA0905900);the Science and Technology Commission of Shanghai Municipality(No.18DZ2293500);the Shanghai Engineering Research Center for Model Organisms(No.19DZ2280500);the Science and Technology Commission of Shanghai Municipality(Nos.22140900102 and 201409001900);the Shanghai Laboratory Animal Research Center(No.2023NS01).
The Gt(ROSA)26Sor(ROSA26)and H11 loci are commonly used as safe harbors for the construction of targeted transgenic mice.However,it remains unclear whether these two loci have distinct effects on transgene expression....
关键词:transgenic mouse knockin Gt(ROSA)26Sor H11 gene expression 
EXPRESS
《China Pictorial》2024年第11期4-5,共2页
Stepping into Caravaggio's World The Caravaggio's World of Art exhibition opened to the public at the National Museum of China in Beijing on November 8,2024.Caravaggio,born in the second half of the 16th century,was a...
关键词:ITALIAN EMOTIONAL EXHIBITION 
The Expression Characteristics and Potential Functions of Heat Shock Factors in Diatom Phaeodactylum tricornutum
《Phyton-International Journal of Experimental Botany》2024年第10期2583-2596,共14页Yanhuan Lin Jiaxin Feng Hao Fang Wei Huang Kanglie Guo Xiyan Liu Shuqi Wang Xiaojuan Liu 
fundings of educational and scientific research projects for young and middle-aged teachers in Fujian Province(Grant number:2022JAT220693);Natural Science Foundation of Guangdong Province(Grant numbers:2024A1515013029 and 2022A1515012141).
Heat shock transcription factor(HSF)are essential regulators of heat shock protein(HSP)gene expression in plants and algae,contributing to their resilience against biotic and abiotic stresses.However,the localization,...
关键词:Diurnal cycle heat shock factor DIATOM cellular homeostasis differentially expressed gene 
EXPRESS
《China Pictorial》2024年第9期4-5,共2页
Video Game's Rave Reviews.The video game"Black Myth:Wukong,"deeply rooted in Chinese culture,received an overwhelmingly positive reaction from players worldwide after its release on August 20.Within 10 hours of its of...
关键词:RELEASE EXPRESS globally 
Evaluating service quality of express logistics service based on online reviews using LDA-LSTM
《Journal of Management Science and Engineering》2024年第3期308-327,共20页Jiahe Hou Weihua Liu Yuenan Cao Siyu Wang Ou Tang 
supported by Major Program of the National Social Science Foundation of China(Grant No.22&ZD139);This research was also funded by Tianjin Science and Technology Planning Project(Grant No.22ZLGCGX00060).The reviewers'comments are also highly appreciated.
The quality of logistics services affects the performance and competitiveness of express logistics companies.Conventional evaluation methods of service quality are based on constructing a rating index system and then ...
关键词:Scraping of reviews LDA model and service elements Sentiment analysis Excellent rating Logistics service 
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