利用染色体片段代换系定位水稻叶片形态性状QTL  

QTL Mapping for Leaf Morphological Traits of Rice Using Chromosome Segment Substitution Lines

作　　者：周勇[1] 陶亚军 姚锐 李畅 谭文琛 裔传灯[1] 龚志云[1] 梁国华[1] ZHOU Yong;TAO Ya-Jun;YAO Rui;LI Chang;TAN Wen-Chen;YI Chuan-Deng;GONG Zhi-Yun;LIANG Guo-Hua(Key Laboratory of Plant Functional Genomics of the Ministry of Education / College of Agriculture, Yangzhou University / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou 225009, China)

机构地区：[1]扬州大学农学院/植物功能基因组学教育部重点实验室/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《作物学报》2017年第11期1650-1657,共8页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2013CBA01405);国家自然科学基金项目(31471458);江苏省重点研发计划(BE2015341);扬州市农业前瞻性研究项目(YZ2014165);上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-1号];江苏高校优势学科建设工程项目资助~~

摘　　要：水稻叶片形态是理想株型的重要组成部分,控制叶片形态基因的挖掘对于塑造水稻理想株型,实现水稻超高产目标具有重要意义。本研究利用广陆矮4号为受体亲本,日本晴为供体亲本构建的一套染色体片段代换系,对水稻上三叶(倒一叶、倒二叶和倒三叶)形态性状与单株籽粒产量进行了相关性分析,并开展了相关QTL定位。结果表明,除剑叶宽外,水稻上三叶的叶长、叶宽都与单株产量呈极显著正相关。同时,通过单因素方差分析和Dunnett’s多重比较,在两年间重复检测到20个控制叶形的QTL,其中叶长QTL 13个(8个表现正向效应,5个表现负向效应);叶宽QTL 7个(4个表现正向效应,3个表现负向效应)。这些QTL的鉴定为水稻叶形性状的分子改良提供了重要遗传信息。Leaf morphology is an important component of ideal plant architecture in rice.Therefore,identification of genes associated with leaf morphologic traits is helpful to shape rice ideal architecture and reach the aim of super high-yield.A set of chromosome segment substitution lines(CSSLs)derived from Guanglu'ai4(recipient)and Nipponbare(donor)were employed to detect quantitative trait loci(QTL)for the length and width of top three leaves(flag leaf,the second and third leaf from top).We also examined the correlation between the leaf traits and grain yield per plant,indicating that except for flag leaf width,other leaf traits were significantly and positively correlated with grain yield per plant.One-way ANOVA and Dunnett's test were used to detect QTLs related to size of rice top three leaves.Thirteen and seven QTLs,for leaf length and width,respectively,were identified.Among them,eight and four QTLs had positive effects,respectively.Identification of these QTLs provides useful information for improving rice leaf morphologic traits.

关 键 词：水稻 叶片形态 染色体片段代换系 QTL 

分 类 号：S511[农业科学—作物学]