对羟基苯甲醛对脑缺血/再灌注损伤的保护作用研究  

The protective effects of 4-hydroxybenzaldehyde on cerebral ischemia/reperfusion injury

作　　者：周晓楠[1] 宋雪兰[1] 李艳 宋波[1] 杨建光 何芳雁[1] ZHOU Xiao nan;SONG Xue lan;LI Yan;SONG Bo;YANG Jian guang;HE Fang yan(Dept of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming650500, China)

机构地区：[1]云南中医学院基础医学院药理学教研室,云南昆明650500

出　　处：《中国药理学通报》2017年第12期1729-1735,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81560664);云南省应用基础研究项目(No 2016FA035);云南省教育厅资助项目(No 2017ZZX287)

摘　　要：目的探讨天麻酚性成分4-HBd对脑缺血/再灌注损伤的保护作用及机制。方法复制大鼠脑缺血/再灌注损伤模型,各组动物脑缺血2 h,再灌注24 h,以神经病学评分、脑梗死体积评价4-HBd对脑缺血/再灌注损伤的保护作用;化学法(连二亚硫酸钠)复制原代皮层神经元OGD/Rep损伤模型,检测细胞存活率、MDA含量、SOD活性、NO释放量以及Bax、Bcl-2、caspase-3蛋白的表达,探讨其脑保护作用的可能机制。结果 4-HBd可降低MCAO/R模型大鼠的神经病学评分(P<0.05)、脑梗死体积(P<0.01),提高OGD/Rep损伤原代皮层神经元的细胞存活率(P<0.05)、SOD的活性(P<0.05),降低模型细胞MDA的含量(P<0.05)、NO的释放量(P<0.05),并能下调模型细胞Bax的表达水平(P<0.05)、上调Bcl-2的表达水平(P<0.05),下调Bax/Bcl-2的比值(P<0.05),最终下调caspase-3的表达水平(P<0.05)。结论 4-HBd对脑缺血/再灌注损伤有保护作用,其机制可能与提高SOD活性、降低MDA含量、减少NO释放、下调Bax蛋白表达、上调Bcl-2蛋白表达、下调caspase-3蛋白表达,进而对抗神经细胞凋亡有关。AimTo verify the mechanisms underlying the protective effects of the4hydroxybenzaldehyde(4HBd)on cerebral ischemia/reperfusion in vivo and in vitro.MethodsThe model of focal cerebral ischemia/reperfusion in rats was established by the suture occlusion method.After reperfusion,neurological score and cerebral infarction rate were assessed.Meanwhile,after pretreatment with4HBd for24h,the primary cultured cortical neuron cells were exposed to oxygen glucose deprivation/reoxygenation(OGD/Rep).The survival rate,MDA,SOD,NO,Bax,Bcl2and caspase3concentrations were assessed.ResultsPretreatment with4HBd significantly decreased neurological score(P<005)and cerebral infarction rate(P<001)in the cerebrum after MCAO/R injury.Furthermore,4HBd significantly increased cell survival rate(P<005),decreased MDA content(P<005),increased SOD activity(P<005),decreased NO levels(P<005),decreased caspase3(P<005),increased Bcl2(P<005)but decreased Bax(P<005)levels and therefore increased Bcl2/Bax ratio in the cerebrum after OGD/R injury.Conclusions4HBd has significant protective effect on MCAO/R rats,and the mechanism may be related to decreasing NO levels,Bax,Bcl2,caspase3and reducing neuronal apoptosis.

关 键 词：对羟基苯甲醛 天麻 脑缺血/再灌注损伤 连二亚硫酸钠 缺糖缺氧/复糖复氧 原代皮层神经元 细胞凋亡 

分 类 号：R-332[医药卫生] R284.1