细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞脂肪变性过程中的表达变化  

Expression changes of cell cycle regulatory genes,Bcl-2,and c-Jun in the process of human hepatic steatosis

作　　者：包成[1,2] 毕玉晶 杨瑞馥[2] 智发朝[1] BAO Cheng;BI Yujing;YANG Ruifu;ZHI Fachao(Nanfang Hospital, Southern Medical University, Guangzhou 510515, China)

机构地区：[1]南方医科大学南方医院.广东省南方消化病研究所,广州510515 [2]北京军事医学科学院微生物流行病研究所

出　　处：《山东医药》2017年第37期12-14,共3页Shandong Medical Journal

基　　金：国家高技术研究发展计划(863计划)(2015AA020702)

摘　　要：目的观察细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞L02脂肪变性过程中的表达变化。方法以油酸和软脂酸(2∶1)混合液处理L02细胞0、5、10、20 h,建立人肝细胞脂肪变性模型。用油红O染色检测L02细胞内脂质含量;用荧光定量PCR技术检测L02细胞脂肪变性过程中CDK2、CDK4、Cyclin D1、Bcl-2、c-Jun mRNA表达。结果随造模时间延长,L02细胞内橘红色脂滴聚集越来越多,至造模20 h,肝细胞染成橘红色。L02细胞内CDK2 mRNA相对表达量逐渐升高,造模5 h达最高(P均<0.05),随后逐渐下降;CDK4 mRNA相对表达量逐渐升高,造模10 h达最高(P均>0.05),随后逐渐下降,造模20 h最低(P均>0.05);不同时间点Cyclin D1 mRNA相对表达量比较差异无统计学意义(P均>0.05);Bcl-2 mRNA相对表达量随造模时间延长而升高,造模10 h达最高(P<0.05),随后降低;造模5、10、20 h的c-Jun mRNA相对表达量均较造模0 h高(P均<0.05),但无变化规律。结论人肝细胞L02脂肪变性过程中癌症相关基因CDK2、CDK4、Bcl-2、c-Jun表达先升高后降低,Cyclin D1表达变化不明显。Objective To investigate the expression changes of cell cycle regulatory genes[Cyclin dependent kinase2(CDK2),Cyclin dependent kinase4(CDK4),and CyclinD1],B cell lymphoma/leukemia2(Bcl2),and c Jun in the process of human hepatic steatosis.Methods L02liver cell line was incubated with mixture of oleic acid and palmitic acid by the ratio of2:1(OP21)for0,5,10,and20h to build the models of human hepatocyte steatosis.Oil Red O staining was performed to evaluate the fat content of the L02liver cells.The mRNA expression levels of CDK2,CDK4,CyclinD1,Bcl2,and c Jun in the L02cells at different time points were detected by real time PCR.ResultsMore and more orange droplets were accumulated in the L02cells with the time goes on,and the liver cells were dyed orange at20h.The mRNA expression level of CDK2increased gradually,reached the peak at5h(P<0.05),and then decreased gradually;the mRNA expression level of CDK4increased gradually,reached the peak at10h(P>0.05),then decreased gradually,and reached the lowest point at20h(P>0.05);no statistically significant difference was found in the expression levels of Cyclin D1at different time points during the process(all P>0.05);the mRNA expression level of Bcl2increased over time,reached the peak at10h(P<0.05),and then decreased;the mRNA expression level of c Jun was higher at5,10,and20h than that at0h(all P<0.05),but there were no regular changes.ConclusionDuring the process of hepatic steatosis,the relative mRNA levels of the cancer related genes(CDK2,CDK4,Bcl2,and c Jun)first increase and then decrease,while the expression of Cyclin D1does not change significantly.

关 键 词：肝细胞脂肪变性 细胞周期 细胞周期蛋白 细胞周期蛋白依赖性激酶 细胞周期蛋白依赖性激酶抑制因子 B细胞白血病-淋巴瘤基因2 C-JUN基因 

分 类 号：R575[医药卫生—消化系统]