姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响  

Effects of curcumin on cell proliferation,aggregation,and chemosensitivity of cervical cancer stem cells

作　　者：张燕华 高艳娥[2] 臧睿 杨红梅 ZHANG Yanhua;GAO Yan-e;ZANG Rui;YANG Hongmei(Xi'an 630 Hospital, Xi'an 710000, China)

机构地区：[1]西安630医院,西安710000 [2]西安交通大学第二附属医院

出　　处：《山东医药》2017年第37期15-17,共3页Shandong Medical Journal

基　　金：陕西省自然科学基金资助项目(2016JM8067)

摘　　要：目的探讨姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响。方法用流式细胞术从人宫颈癌细胞株CASKI中分选CD133+的宫颈癌干细胞。将宫颈癌干细胞随机分为七组,CC10组、CC20组、CC40组、CC60组、CC80组及CC100组分别给予终浓度为10、20、40、60、80、100 mg/m L姜黄素进行培养,对照组给予等量溶剂与培养基培养。用MTT法检测各组细胞增殖抑制率。取对照组、CC60组细胞用无血清培养基培养1周,光镜下计数聚集的直径大于100μm的干细胞球个数,流式细胞术检测宫颈癌干细胞比例。取宫颈癌干细胞随机分为BV组、CC组、BV+CC组、对照组四组,分别加入10 mg/m L贝伐单抗、60 mg/m L姜黄素、10 mg/m L BV+60 mg/m L姜黄素、等量溶剂与培养基,培养48 h后采用流式细胞术测算细胞凋亡率。结果 CC10组、CC20组、CC40组、CC60组、CC80组、CC100组细胞增殖抑制率均高于对照组(P均<0.05);且随着姜黄素浓度的增加,宫颈癌干细胞增殖抑制率逐渐升高(P均<0.05)。CC60组直径大于100μm的干细胞球个数少于对照组(P<0.05),干细胞比例低于对照组(P<0.05)。BV+CC组细胞凋亡率高于对照组、BV组、CC组(P均<0.05),CC组高于对照组、BV组(P均<0.05),BV组高于对照组(P均<0.05)。结论姜黄素可抑制宫颈癌干细胞增殖,降低其聚集能力,增加其对贝伐单抗的治疗敏感性。ObjectiveTo explore the effects of curcumin on the cell proliferation,aggregation,and the sensitivity to bevacizumab of cervical cancer stem cells.Methods The CD133+cervical cancer stem cells(CCSCs)were isolated from CASKI cells by flow cytometry.The CCSCs were divided into seven groups:CC10group,CC20group,CC40group,CC60group,CC80group,and CC100group which were given a final concentration of10,20,40,60,80and100mg/mL curcumin,while cells in the control group were given the same amount of solvent and culture medium.The inhibition rate of cell proliferation in each group was detected by MTT.The cells in the control group and CC60group were cultured in serum free medium for1week.The number of stem cell spheres with diameter greater than100μm was counted by light microscope,and the proportion of CD133+cells was detected by flow cytometry.The cervical cancer stem cells were divided into four groups,BV group(added with bevacizumab10mg/mL),CC group(added with60mg/mL curcumin),BV+CC group(added with10mg/mL bevacizumab and60mg/mL curcumin),and the control group(treated with the same amount of solvent and medium).After48hour culture,the apoptosis rate was determined by flow cytometry.ResultsThe rates of cell proliferation inhibition in the CC10group,CC20group,CC40group,CC60group,CC80group,and CC100group were higher than that of the control group(all P<0.05),and with the increase of curcumin concentration,the proliferation inhibition rate of cervical cancer stem cells gradually increased(all P<0.05).The number of stem cell spheres with diameter greater than100μm of the CC60group was less than that of the control group(P<0.05).The proportion of cervical cancer stem cell in the CC60group was lower than that in the control group(P<0.05).The apoptosis rate in the BV+CC group was higher than that in the control group,BV group,and CC group(P<0.05),the apoptosis rate in the CC group was higher than that in the control group and BV group(P<0.05),and the apoptosis rate in the BV group was higher than that in the control group(P<0.0

关 键 词：宫颈癌 宫颈癌干细胞 姜黄素 细胞增殖 细胞聚集 贝伐单抗 治疗敏感性 

分 类 号：R737.33[医药卫生—肿瘤]