儿童急性B淋巴细胞白血病(B-ALL)调节性T淋巴细胞变化及调节机制分析  

Analysis on the changes of regulatory T lymphocytes in children with acute B lymphocytic leukemia(B-ALL) and its regulatory mechanism

作　　者：张小薇[1] 任建敏[1] 傅丽敏[1] 周灵玲[1] ZHANG Xiaowei;REN Jianmin;FU Limin;ZHOU Lingling(Clinical Laboratory, Lishui Central Hospital in Zhejiang Province, Lishui 323000, China)

机构地区：[1]浙江省丽水市中心医院检验科,浙江丽水323000

出　　处：《中国现代医生》2017年第31期5-8,共4页China Modern Doctor

基　　金：浙江省自然科学基金项目(LQ17H080001)

摘　　要：目的分析儿童急性B淋巴细胞白血病(B-ALL)调节性T淋巴细胞变化及调节机制。方法在2016年2月23日~2017年2月23日期间选取50例健康体检者(对照组)、50例急性B淋巴细胞白血病患儿(观察组)为实验对象,对两组受检者均进行分离外周血CD4^+T细胞、荧光定量PCR(RT-PCR、REAL-TIME PCR、总RNA定量和提取、RT-PCR产物鉴定)、酶联免疫吸附试验、流式细胞术检测,随后对比两组受检者各项检测结果。结果观察组患儿的CD28/CD4^+Foxp3^+(524.86±28.42)、ICOS/CD4^+CD25^(high)Foxp3^+(725.69±15.74)、TGF-βRII/CD4^+(61.75±10.08)、Mtge-β(58.44±10.85)、IL-35p35(326.84±35.46)、IL-35EB13(128.56±12.74)、TGF-β(81.46±5.43)、IL-10(135.75±12.75)、ICOS^+/CD4^+CD25^(high)Foxp3(3.86±1.75)%、Foxp3(812.43±56.32)、CD4^+CD25^(high)Foxp3(9.85±2.45)%均高于对照组健康体检者的CD28/CD4^+Foxp3^+(158.33±21.06)、ICOS/CD4^+CD25^(high)Foxp3^+(352.12±10.03)、TGF-βRII/CD4^+(28.42±5.89)、Mtge-β(23.17±5.33)、IL-35p35(154.28±26.31)、IL-35EB13(48.31±6.52)、TGF-β(29.51±6.38)、IL-10(24.91±5.22)、ICOS^+/CD4^+CD25^(high)Foxp3(2.45±0.85)%、Foxp3(322.41±18.75)、CD4^+CD25^(high)Foxp3(4.12±1.75)%(P<0.05)。结论导致急性B淋巴细胞白血病患儿出现亚群比例失调的主要原因为转化生长因子-β(transforming growth factor-β,TGF-β)、分化群28(Cluster of Differentiation 28)、ICOS过度活化。Objective To analyze the changes and regulatory mechanism of regulatory T lymphocytes in children with acute B lymphocytic leukemia(B-ALL).Methods A total of50children of healthy subjects(control group)and50children with acute B lymphocytic leukemia(observation group)who were selected from February23rd,2016to February23rd,2017were selected as the study subjects.CD4+T cells were isolated from peripheral blood in both groups,and quantitative PCR(RT-PCR,REAL-TIME PCR,total RNA quantification and extraction,RT-PCR product identification),enzyme-linked immunosorbent assay,and flow cytometry were carried out,and then the results of each test were compared between the two groups.Results CD28/CD4+FOXP3+(524.86±28.42),ICOS/CD4+CD25high FOXP3+(725.69±15.74),TGF-βRII/CD4+(61.75±10.08),Mtge-β(58.44±10.85),IL-35p35(326.84±35.46),IL-35EB13(128.56±12.74),TGF-β(81.46±5.43),IL-10(135.75±12.75),ICOS+/CD4+CD25high Foxp3(3.86±1.75)%,Foxp3(812.43±56.32),CD4+CD25high Foxp3(9.85±2.45)%in the observation group were all higher than CD28/CD4+FOXP3+(158.33±21.06),ICOS/CD4+CD25high FOXP3+(352.12±10.03),TGF-βRII/CD4+(28.42±5.89),Mtge-β(23.17±5.33),IL-35p35(154.28±26.31),IL-35EB13(48.31±6.52),TGF-β(29.51±6.38),IL-10(24.91±5.22),ICOS+/CD4+CD25high Foxp3(2.45±0.85)%,Foxp3(322.41±18.75),CD4+CD25high Foxp3(4.12±1.75)%in the healthy subjects in the control group(P<0.05).Conclusion The main reason for subgroup ratio imbalance in children with acute B lymphocytic leukemia is the over-activation of transforming growth factor-β(TGF-β),cluster of differentiation28and ICOS.

关 键 词：急性B淋巴细胞白血病 T淋巴细胞 调节机制 酶联免疫吸附试验 

分 类 号：R73[医药卫生—肿瘤]