量子点-卟啉纳米复合体系在肿瘤细胞成像中的应用  

Application of CdTe QDs-TMPyP Nanocomplex in Imaging of Tumor Cells

作　　者：胡先运[1,2,3] 孟铁宏 黄星宏[1] 张汝国 江家志[1] 刘瑛 朱楠[1] HU Xianyun;MENG Tiehong;HUANG Xinghong;ZHANG Ruguo;JIANG Jiazhi;LIU Ying;ZHU Nan(Qiannan Medical College for Nationalities ,Duyun 558000, Guizhou, P. R. China;State Key Laboratory of Bioelectronics , School of Biological Science and Medical Engineering,Southeast University , Nanjing 210096, Jiangsu, P. R. China;The Key Laboratory of Chemistry fo r Natural Products of Guizhou Province and Chinese Academy of Sciences , Guiyang 550002, Guizhou, P. R. China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558000 [2]东南大学生物科学与医学工程学院生物电子学国家重点实验室,江苏南京210096 [3]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《影像科学与光化学》2017年第6期853-860,共8页Imaging Science and Photochemistry

基　　金：国家自然科学基金项目(21545006);贵州省自然科学基金(2015GZ48861;2016GZ13752);中央高校基本科研业务费专项资金(KYLX0187);黔南州优青项目;黔南科技计划项目(黔南科合工字[2016]12号;黔南科合社字[2016]1号);黔南民族医专科研基金(QNYZ201601;QNYZ201605)资助

摘　　要：本文合成了高荧光量子产率、单分散性好的水溶性CdTe量子点(quantum dots,QDs),并与α,β,γ,δ-四(1-甲基吡啶嗡-4-基)卟吩对甲苯磺酸盐(TMPyP)组装成QDs-TMPyP纳米复合物,研究了该复合物检测DNA的机理以及肿瘤细胞成像。结果显示,QDs-TMPyP纳米复合物通过光致电子转移机制检测DNA,当CdTe QDs和CdTe QDs-TMPyP浓度低于1.0μmol/L时,HeLa肿瘤细胞存活率达92%以上,表现出低的细胞毒性。0.2μmol/L CdTe QDs-TMPyP作用于肿瘤细胞时,细胞生长状态良好,对细胞内能谱分析发现细胞内含有Cd和Te原子。CdTe QDs-TMPyP复合物比CdTe QDs更易被HeLa细胞摄取,利用量子点荧光成功实现了细胞核内成像,为宫颈癌细胞药物输送和细胞成像的深入研究打下基础。The solution CdTe quantum dots(Dots)with high quantum yield and good homogeneous distribution conjugate meso-tetrakis(N-methylpyridinium-4-yl)porphyrin(TMPyP)to construct QDs-TMPyP nanocomplex.The QDs-TMPyP nanocomplex was achieved to detection of DNA by photo induced electrontransfer(PIET)process.CdTe QDs and CdTe QDs-TMPyP showed a remarkable inhibition for the proliferation of cancer cells in vitro at higher concentrations,but only small effects at lower concentrations.The cells viabilities of HeLa cells were above92%under treated with lower than1.0pm ol/L CdTe QDs and CdTe QDs-TMPyP for24:h.The HeLa cancer cells treated with0.2pm ol/L CdTe QDs-TMPyP were found to contain Cd and Te atoms by EDS analysis.The uptake of CdTe QDs-TMPyP was easier than CdTe QDs in HeLa cancer cells.The CdTe QDs-TMPyP nanocomplex was successfully achieved in HeLa cells fluorescent imaging,which lays the foundation for the drug delivery and cell imaging.

关 键 词：量子点 α β γ δ-四(1-甲基吡啶嗡-4-基)卟吩对甲苯磺酸盐 荧光成像 DNA 

分 类 号：O657.3[理学—分析化学] R73-3[理学—化学] TB383.1[医药卫生—肿瘤]