大蒜素对大鼠心房肌细胞瞬时外向钾电流的影响  

Effects of Allicin on Transient Outward Potassium Current in Rat Atrial Myocytes

作　　者：陈晨[1] 王瑞 蔡忠琦 杨易 林琨[3] 刘昱圻[3] 杨洁[3] 李泱[3] CHEN Chen;WANG Rui;CAI Zhongqi;YANG Yi;LIN Kun;LIU Yuqi;YANG Jie;LI Yang(Department of Cardiology, Beijing Anzhen Hospital,Capital Medical University, Beijing 100029,China;Department of Emergency, Xi ' an Air Force Hospital,Xi, an 710054,China;Department of Cardiology,Chinese PLA General Hospital, Beijing 100853, China)

机构地区：[1]首都医科大学附属北京安贞医院心内科,北京100029 [2]西安空军医院急诊科,西安710054 [3]解放军总医院心内科,北京100853

出　　处：《医药导报》2017年第11期1231-1235,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(81170177)

摘　　要：目的观察大蒜素对大鼠单个心房肌细胞钾电流的作用。方法灌流酶法分离大鼠单个心房肌细胞,细胞外局部灌流法给药,采用全细胞膜片钳技术记录大鼠单个心房肌细胞瞬时外向钾电流(Ito)。结果在+50 m V电压下,30μmol·L-1大蒜素可使大鼠心房肌细胞Ito峰值由(20.5±2.2)p A/p F降低至(11.3±2.1)p A/p F(P<0.01,n=12),电流-电压曲线下移,该过程呈浓度依赖性和电压依赖性特点,半数抑制浓度(IC50)为(19.0±2.5)μmol·L-1。门控动力学研究显示,大蒜素可使Ito通道稳态激活曲线右移,恢复时间延长导致激活过程减慢,且失活后再激活延迟。结论大蒜素可通过抑制通道的激活及失活后的恢复而降低心房肌细胞Ito。ObjectiveTo study the effect of allicin(All)on potassium current in single atrial myocytes in rats.MethodsIsolated rat atrial myocytes were isolated by perfusion and administered by extracellular perfusion method.Whole cell patch clamp technique was used to record transient outward potassium current(Ito)in atrial myocyte.ResultsIn presence of30μmol·L-1of All,the peak current of Ito was significantly reduced from(20.5±2.2)pA/pF to(11.3±2.1)pA/pF at+50mV of test potential(P<0.01,n=12).This effect of All showed voltage and concentration dependence with IC50of(19.0±2.5)μmol·L-1.The steady state activation curve of Ito was shifted to more positive potential and recovery time from inactivation was prolonged.In addition,they fail to find any effect of All on the steady state inactivation and closed state inactivation of Ito.ConclusionAll can inhibit Ito by slowing down the process of activation and recovery from inactivation of channel.

关 键 词：大蒜素 心房肌细胞 瞬时外向钾电流 膜片钳 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]