MYLPF基因在肉牛肌肉生长过程中的功能研究  

Study on the Function of MYLPF Gene of Muscle Growth in Cattle

作　　者：刘瑞莉[1,2] 康晓晨 吴磊[1,2] 袁玮 董雅娟 LIU Rui li;KANG Xiao chen;WU Lei;YUAN Wei;DONG Ya juan(Animal Embryo Engineering Center,Qingdao Agricultural University, Qingdao 266109,China;Shandong Black Cattle Breeding Engineering Technological Research Center,Qingdao 266109,China;Shandong Black Cattle Science Technology Co.,Ltd., Zibo 256306,China)

机构地区：[1]青岛农业大学,动物胚胎工程中心,青岛266109 [2]山东省黑牛繁育工程技术研究中心,青岛266109 [3]山东布莱凯特黑牛科技股份有限公司,淄博256306

出　　处：《中国畜牧兽医》2017年第11期3236-3242,共7页China Animal Husbandry & Veterinary Medicine

基　　金：山东省现代农业产业技术体系牛产业创新团队建设(668/2216030)

摘　　要：试验旨在探究快肌肌浆球蛋白可调节磷酸化轻链(myosin light chain,phosphorylatable,fast skeletal muscle,HUMMLC2B或MYLPF)基因在布莱凯特黑牛各组织中的表达情况,以及布莱凯特黑牛与鲁西黄牛背最长肌中MYLPF基因表达量的变化规律,为探讨其与肌肉生长发育关系奠定基础。以布莱凯特黑牛和鲁西黄牛为研究对象,利用实时荧光定量PCR检测其背最长肌、心脏、肺脏、肝脏、脾脏等10个组织中MYLPF基因的表达情况,并对布莱凯特黑牛和鲁西黄牛2、6、10和12月龄4个不同时期背最长肌中MYLPF基因的表达量变化进行研究。结果表明,MYLPF基因在背最长肌中高表达,在心脏中少量表达,在其他组织中几乎不表达。MYLPF基因在2、6、10和12月龄牛背最长肌中均高表达,但随着年龄的增加MYLPF基因的表达量逐渐降低,2月龄的表达量最高,极显著高于其他3个时期(P<0.01),12月龄表达量最低。4个时期布莱凯特黑牛背最长肌中MYLPF基因的表达量均高于鲁西黄牛,其中10月龄差异极显著(P<0.01),2和12月龄差异显著(P<0.05),6月龄差异不显著(P>0.05)。综上结果表明,背最长肌中MYLPF基因可能对骨骼肌生长发育起调节作用,可为研究肉牛骨骼肌生长发育机理和阐明肌肉生长的分子机制提供参考。This study was aimed to investigate the expression level of myosin light chain,phosphorylatable,fast skeletal muscle(MYLPF)gene in the tissues of Black cattle,and its expression level in the longissimus dorsi muscle of Black cattle and Luxi cattle,and to lay the foundation for the study of its relationship with muscle growth and development.The MYLPF gene expression level in10tissues of longissimus dorsi,heart,lung,liver and spleen and so on were detected by Real time quantitative PCR,and then the changes of MYLPF gene expression level in longissimus dorsi of Black cattle and Luxi cattle at2,6,10and12months of age were studied.The results showed that MYLPF gene was highly expressed in the longissimus dorsi,lowly expressed in the heart,and the other tissues were almost not expressed.The MYLPF gene expression level in the longissimus dorsi was high in2,6,10and12months,with the increase of age,the MYLPF gene expression level decreased gradually,the expression level of2months of age was the highest,which was extremely significantly higher than that of the other three periods(P<0.01).The MYLPF gene expression level in the longissimus dorsi of Black cattle was higher than that of Luxi cattle in four period,which in10months of age were extremely significantly different(P<0.01),2and12months of age had significant difference(P<0.05),the difference in6months of age was not significant(P>0.05).The results suggested that MYLPF gene in the longissimus dorsi might play a regulatory role in the growth and development of skeletal muscle,which might provide a reference for studying the mechanism of growth and development of skeletal muscle and the molecular mechanism of muscle growth.

关 键 词：布莱凯特黑牛 鲁西黄牛 MYLPF基因 背最长肌 

分 类 号：S823.92[农业科学—畜牧学]