DNMT3B基因启动子区-579G>T多态性与江苏宿迁地区人群胃癌易感性的分析  

Study on the relationship between promoter -579G>T of DNMT3B with genetic susceptibility to gastric cancer in Jiangsu Suqian region

作　　者：邱巍[1] 赵建江[1] 陈素梅[1] 刘东声[1] QIU Wei;ZHAO Jianjiang;CHEN Sumei;LIU Dongsheng(Department of Clinical Laboratory,People′s Hospital of Suqian City,Suqian,Jiangsu 223800,China)

机构地区：[1]宿迁市人民医院检验科,江苏宿迁223800

出　　处：《国际检验医学杂志》2017年第20期2842-2844,共3页International Journal of Laboratory Medicine

基　　金：江苏省宿迁市科技局资助项目(S201208)

摘　　要：目的分析DNA甲基转移酶3B(DNMT3B)基因启动子区-579G>T单核苷酸多态性(SNP)与江苏宿迁地区汉族人群胃癌(GC)易感性的关系。方法病例组为233例胃癌患者,对照组为208例体检健康者。提取外周血基因组DNA,利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测DNMT3B启动子区-579G>T位点的基因频率分布。结果 TT基因型与GT+GG基因型频率在胃癌组和对照组中差异有统计学意义(χ~2=6.517,P<0.05)。按年龄分组后,年龄≥60岁的胃癌组和对照组人群间比较,TT与GT+GG基因型频率差异有统计学意义(χ~2=4.728,P<0.05)。按性别分组后,2组间基因型频率差异均无统计学意义(χ~2=3.541,P>0.05;χ~2=3.676,P>0.05)。结论 DNMT3B-579G>T与胃癌的遗传易感性有关联,可作为胃癌遗传易感性风险因素。ObjectiveTo investigate the association between DNA methyltransferase3B(DNMT3B)promoter single nucleotide polymorphism(SNP)with susceptibility to gastric cancer(GC)in Han population of Suqian region.MethodsA total of233patients with gastric cancer were recruited into the study group,208healthy persons were recruited in the control group.Genomic DNA was extracted from peripheral blood,and the frequency distribution of-579G>T locus in DNMT3B promoter region was detected by polymerase chain reaction with restriction fragment length polymorphism(PCR RFLP).ResultsThe distribution of TT genotypes compared with GT+GG in all the two groups were significantly different(χ2=6.517,P<0.05).When stratified by age,there was significant difference between TT and GT+GG genotypes at the age range≥60years(χ2=4.728,P<0.05).When stratified by gender,there was no significant difference between different gender groups(χ2=3.541,P>0.05;χ2=3.676,P>0.05).ConclusionThe DNMT3B-579G>T is associated with the genetic susceptibility of gastric cancer.It might be a risk factor of gastric cancer.

关 键 词：DNA甲基转移酶3B 胃癌 单核苷酸多态性 

分 类 号：R735.2[医药卫生—肿瘤]