胃袖状切除术改善2型糖尿病小鼠脂肪组织胰岛素抵抗机制研究  

Effect of sleeve gastrectomy on insulin resistance in adipose tissue of obesity-associated type 2 diabetes(+db/+db) mice

作　　者：赵文嫣[1] 孙明[2] 冯勇[1] 耿东华[1] 刘金钢[3] Wen-yan Zhao;Ming Sun;Yong Feng;Dong-hua Geng;Jin-gang Liu(Department of General Surgery,Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, China;Department of Urology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, China;Department of General Surgery, the Fourth Affiliated Hospital, China Medical University, Shenyang, Liaoning 110032, China)

机构地区：[1]中国医科大学附属盛京医院普通外科,辽宁沈阳110004 [2]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [3]中国医科大学附属第四医院普通外科,辽宁沈阳110032

出　　处：《中国现代医学杂志》2018年第1期16-20,共5页China Journal of Modern Medicine

基　　金：沈阳市科学技术计划项目应用基础研究专项(No:150422);辽宁省自然科学基金(No:2015020505)

摘　　要：目的探讨胃袖状切除术(SG)对2型糖尿病小鼠脂肪组织微囊蛋白1(caveolin-1)表达及胰岛素抵抗的影响。方法实验于2015年1~6月在中国医科大学附属盛京医院中心实验室进行。选择30只肥胖2型糖尿病小鼠(+db/+db,C57BL/KsJ)为研究对象,按体重随机等分为3组:db/db组(无处理)、db/db-SG组(行SG)、db/db-sham组(假手术),每组10只。术前3 d和术后7、14、21及28 d测定动物体重及每100 g体重摄食量。术前5 d和术后30 d,测定小鼠空腹血糖(FBG)及口服葡萄糖耐量(OGTT),ELISA检测血浆胰岛素、三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白(LDL);Western blot和实时聚合酶链反应(Real-time PCR)检测内脏脂肪组织caveolin-1、GLUT4蛋白及mRNA表达。结果 db/db-SG组小鼠术后体重、摄食量、空腹血糖、OGTT曲线下面积、胰岛素抵抗指数与db/db组及db/db-sham组比较,差异有统计学意义(P<0.05),db/db-SG组小鼠术后体重及摄食量降低,空腹血糖下降,OGTT曲线下面积减少,胰岛素抵抗指数下降;db/db-SG组血清TG、TC、LDL水平与db/db组及db/db-sham组比较,差异有统计学意义(P<0.05),db/db-SG组小鼠下降;db/db-SG组小鼠脂肪组织caveolin-1、GLUT4蛋白和mRNA表达与db/db组及db/db-sham组比较,差异有统计学意义(P<0.05),均升高。结论 SG可在术后短期内有效降低2型糖尿病小鼠体重及摄食量,降低TG、TC,改善胰岛素抵抗,可能与脂肪组织caveolin-1及GLUT4水平下降有关。Objective To observe the effect of sleeve gastrectomy(SG)on the expression of caveolin-1in adipose tissue and insulin resistance status.Methods Thirty10-week old obesity-associated type2diabetes(+db/+db)(C57BL/KsJ)mice were randomly allocated to db/db-SG group,db/db-sham group and db/db blank control group,each group had10rats.Body weight and food intake were recorded3d before operation and7,14,21and28d after operation.On the5th d before operation and the30th d after operation,FBG and OGTT were detected before and after operation;ELISA was used to detect the plasma insulin,TG,TC and LDL;RT-PCR and Western blot were used to detect the expressions of caveolin-1and GLUT4in the viceral adipose tissue.Results Compared with the db/db-sham group and the blank control group,the mice in the SG group showed obviously-reduced body weight and food intake(P<0.05),apparent downward trends of FBG level,AUC of OGTT,and HOMA-IR(P<0.05).The plasma TC,TG and LDL levels in the SG group decreased as well(P<0.05).The expressions of caveolin-1and GLUT4protein and mRNA in the viceral adipose tissue significantly increased in the SG group compared to the db/db-sham group and the blank control group(P<0.05).Conclusions SG can significantly lower the body weight and food intake,decrease the plasma TC,TG and LDL,and alleviate the insulin resistance status in a short period after surgery,which may be related to reduced expression of caveolin-1and GLUT4in the adipose tissue.

关 键 词：袖状胃切除 脂肪组织 胰岛素抵抗 脂肪组织微囊蛋白1 

分 类 号：R-332[医药卫生]