靶向沉默c-fos基因表达对胶质瘤U87MG细胞增殖与侵袭的影响  

Effects of c-fos targeted silence on proliferation and invasiveness of human glioma cell U87

作　　者：桂志勇 冯军[2] 黄俊红 白敬洋 GUI Zhi-yong;FENG Jun;HUANG Jun-hong;BAI Jingyang(Department of Neurosurgery, the First people's Hospital of Luohe City, Luohe 462000, China;Department of Neurosurgery, Affiliated Union Hospital, Tongji Medical School, Huazhong University of Sciences andTechnology, Wuhan 430022, China)

机构地区：[1]漯河市第一人民医院神经外科,河南462000 [2]华中科技大学同济医学院附属协和医院神经外科,武汉430022

出　　处：《中国临床神经外科杂志》2017年第12期834-838,共5页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨靶向沉默c-fos基因表达对胶质瘤U87MG细胞体外增殖与侵袭的影响。方法构建慢病毒载体c-fos-sh RNA,将其转染人脑胶质瘤U87MG细胞,同时将空载体转染细胞作为空载对照,未转染细胞作为空白对照;观察U87MG细胞形态、c-fos m RNA和蛋白表达变化、细胞侵袭和迁移能力、细胞生存率和细胞凋亡率。结果 c-fos-sh RNA慢病毒转染U87MG细胞48 h后,荧光显微镜下观察发现空白对照组U87MG细胞无绿色荧光,而空载体组和转染组U87MG细胞可见绿色强荧光和清晰的细胞轮廓。与空白对照组、空载体组相比,转染组U87MG细胞c-fos m RNA和c-fos蛋白表达显著降低(P<0.05);慢病毒转染U87MG细胞24 h后,转染组U87MG细胞存活率显著降低(P<0.05),细胞活力显著减弱(P<0.05);细胞迁移和侵袭能力明显减弱(P<0.05);慢病毒转染72 h后,转染组U87MG细胞凋亡数量显著上升(P<0.05)。结论沉默c-fos基因表达可显著抑制脑胶质瘤U87MG细胞增殖和侵袭能力。Objective To explore the effects of lentivirus-mediated c-fos targeted silence on the proliferation and invasiveness of human glioma cell U87in vitro.Methods Lentiviral vector c-fos-shRNA was transfected into human glioma U87cells.Empty vector-transfected human glioma U87cells served as the no-load control,and non-transfected human glioma U87cells serve as the blank control.The morphology of U87cells,the expression of c-fos mRNA and c-fos protein,the invasiveness and migration ability of U87cells,and the survival rate of glioma cells,and the apoptosis rate of U87cells were observed in all the groups.Results No green fluorescence of U87cells in the blank control group,strong green fluorescence and clear cell outline of U87cells in the no-load group and the transfection group were observed by the fluorescence microscope.The levels of c-fos mRNA and protein expressions were significantly lower in the transfection group48hours after the transfection than those in the no-load and blank control groups(P<0.05)The survival rate,migration and invasion ability of U87cells were significantly lower in the transfection group48hours after the transfection than thsoe in the blank control and no-load groups(P<0.05).The number of apoptotic cells was significantly more in transfection group72hours after the transfection than that in the blank control and no-load groups(P<0.05).Conclusions It is suggested that c-fos targeted silence can significantly inhibit the proliferation and invasiveness of human glioma U87cells,and c-fos-shRNA lentivirus-mediated gene therapy may be targeted as a new candidate for c-fos gene therapy.

关 键 词：胶质母细胞瘤 C-FOS SHRNA 细胞增殖 细胞侵袭 RNA干扰技术 

分 类 号：R739.41[医药卫生—肿瘤] Q789[医药卫生—临床医学]