Diversity of ds RNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA  

作　　者：ZHENG Li LIU Chen ZHANG Meiling YANG Mei ZHOU Erxun 

机构地区：[1]Guangdong Province Key Laboratory of Microbial Signals and Disease Control/College of Agriculture,South China Agricultural University

出　　处：《Rice science》2018年第1期57-60,共4页水稻科学（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China (Grant No. 31470247)

摘　　要：Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing regions.Although several sporadic studies of mycoviruses in R.solani AG-1 IA have been reported for single strain of R.solani AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural populations.In this study,43 R.solani AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral infections.Electrophoretypes showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as viruses.Furthermore,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus,our findings indicate mycoviruses infecting R.solani AG-1 IA in China are diverse,widespread and universal.Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing regions.Although several sporadic studies of mycoviruses in R.solani AG-1 IA have been reported for single strain of R.solani AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural populations.In this study,43 R.solani AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral infections.Electrophoretypes showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as viruses.Furthermore,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus,our findings indicate mycoviruses infecting R.solani AG-1 IA in China are diverse,widespread and universal.

关 键 词：MYCOVIRUS RHIZOCTONIA SOLANI reverse transcription POLYMERASE chain reaction DIVERSITY dsRNA rice sheath blight 

分 类 号：S511[农业科学—作物学]