木质纤维素薄膜制备与酶解过程的QCM-D分析  

QCM-D Analysis of Film Formation and Enzymatic Hydrolysis of Lignocellulose

作　　者：苏荣欣[1,2,3,4] 陈眯眯 黄仁亮 齐崴[1,2,3,4] 王梦凡[1,2] 何志敏 Su Rongxin;Chen Mimi;Huang Renliang;Qi Wei;Wang Mengfan;He Zhimin(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China;State Key Laboratory of Chemical Engineering,Tianjin University,Tianjin 300350,China;Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300350,China;Tianjin Key Laboratory of Membrane Science and Desalination Technology,Tianjin 300072,China)

机构地区：[1]天津大学化工学院,天津300350 [2]化学工程联合国家重点实验室(天津大学),天津300350 [3]天津化学化工协同创新中心,天津300350 [4]天津市膜科学与海水淡化技术重点实验室,天津300072

出　　处：《天津大学学报（自然科学与工程技术版）》2018年第1期1-8,共8页Journal of Tianjin University：Science and Technology

基　　金：国家自然科学基金资助项目(51473115;21276192)~~

摘　　要：耗散型石英晶体微天平(QCM-D)是基于石英晶体的压电效应对其电极表面质量变化进行测量的仪器,已被证明是一种高灵敏的在线表界面过程分析工具.本文综述了近年来木质纤维素薄膜制备及应用QCM-D研究其酶解过程的分析进展,分别从薄膜制备、纤维素酶吸附(纤维素、木质素、混合底物)、酶解历程分析、动力学建模等4个方面进行简要介绍.上述研究成果有助于理解纤维素酶与木质纤维素的相互作用规律,为设计新型复配酶制剂、深刻理解纤维素酶解机制提供基础和指导.Quartz crystal microbalance with dissipation(QCM-D)is an instrument used to measure the mass change of the electrode surface based on the piezoelectric effect of quartz crystal and has proved to be a highly sensitive online interface process analysis tool.This review summarizes the lignocellulose film preparation and the analysis progress of QCM-D in enzymatic hydrolysis in recent years.Four aspects,which include thin film preparation,cellulase adsorption onto cellulose,lignin,and lignocellulosic substrates,enzymatic hydrolysis analysis and dynamics modeling,are briefly introduced.The above research results help to understand the interaction characteristics between lignocellulose and cellulase,providing foundation and guidance for designing a new compounded enzyme system and a profound understanding of the mechanism of cellulose enzymatic hydrolysis.

关 键 词：木质纤维素 纤维素酶 石英晶体微天平 膜 吸附 水解 

分 类 号：Q64[生物学—生物物理学]