脐带间充质干细胞移植急性脑创伤大鼠损伤区域微血管密度的变化  

Umbilical cord mesenchymal stem cell transplantation increases microvessel density in surrounding areas of acute traumatic brain injury in rats

作　　者：徐海环 董化江[1] 商崇智[3] 赵明亮 Xu Hai-huan;Dong Hua-jiang;Shang Chong-zhi;Zhao Ming-liang(School of Precise Instrument and Optoelectronic Engineering, Tianjin University, Tianjin 300072, China;Department of Dermatology, Affiliated Hospital of Logistics University of People's Armed Police Force, Tianjin 300162, China;Key Laboratory of Neural Repair in Tianjin, Tianjin 300162, China)

机构地区：[1]天津大学精密仪器与光电子工程学院,天津市300072 [2]武警后勤学院附属医院皮肤科,天津市300162 [3]天津市神经修复重点实验室,天津市300162

出　　处：《中国组织工程研究》2017年第33期5320-5324,共5页Chinese Journal of Tissue Engineering Research

基　　金：天津市自然科学基金(16JCYBJC27600)~~

摘　　要：背景:脐带间充质干细胞是一种多能干细胞,能够减轻损伤区域的炎性反应,促进微血管再生。目的:探讨脐带间充质干细胞移植对急性颅脑创伤大鼠脑组织微血管密度的影响。方法:30只SD大鼠采用随机数字法分为3组:对照组(未损伤组)、脑创伤模型组和细胞移植治疗组,每组10只。采用自由落体方法复制颅脑创伤模型,经脑室途径输注脐带间充质干细胞1.5×106个。细胞移植后24 h进行神经损伤评分。细胞移植后14 d,采用免疫组化法检测受损脑组织附近微血管密度。结果与结论:(1)与脑创伤模型组比较,细胞移植治疗组微血管密度升高明显(P<0.05);(2)细胞移植治疗组神经损伤评分优于脑创伤模型组(P<0.05);(3)结果表明,脐带间充质干细胞移植治疗急性颅脑创伤大鼠,可有效改善脑损伤区域血管重建,促进受损脑组织神经功能恢复。BACKGROUND:Umbilical cord mesenchymal stem cells(UC-MSCs)are a kind of pluripotent stem cells capable of relieving inflammation and edema in injured areas,promoting microvascular regeneration and improving wound healing.OBJECTIVE:To explore the therapeutic effects of UC-MSCs transplantation on acute traumatic brain injury(TBI)in rats and the effect on microvessel density of the rat brain tissue.METHODS:Thirty Sprague Dawley rats were randomly divided into three groups,control group,TBI model group and UC-MSCs treatment group(n=10per group).TBI models were made in the rats by free fall method.US-MSCs(1.5×106)were infused through the cerebral ventricle in the rats.Neurological severity scores were assessed at24hours after cell transplantation.The microvessel density in the injured brain tissue was detected using immunohistochemistry method at14days after cell transplantation.RESULTS AND CONCLUSION:Compared with the TBI model group,the microvessel density value was significantly increased after UC-MSCs transplantation(P<0.05),and the neurological severity score also became better in the UC-MSCs treatment group(P<0.05).In summary,UC-MSCs transplantation can effectively improve became vascular remodeling and have a good neuroprotection in acute TBI rats.

关 键 词：干细胞 脐带脐血干细胞 脐带间充质干细胞 脑创伤 微血管密度 神经缺损评分 天津市自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]