亮丙瑞林与缩宫素对大鼠子宫内膜异位症模型的疗效比较  

Comparison of the therapeutic effects of oxytocin and leuprorelin on rat endometriosis model

作　　者：乔海风[1] 开海丽 刘颖蕾[1] 曹杨 鲁晓燕[1] 刘曼华[1] QIAO Hai-feng,;KAI Hai-li;LIU Ying-lei;Cao-Yang;Lu-Hai-Yan;Liu-Man-Hua(Department of Obstetrics and Gynecology, Nantong First People 's Hospital, The Second Affiliated Hospital of Nantong University, Nantong Jiangsu 226001, China.)

机构地区：[1]南通市第一人民医院(南通大学第二附属医院)妇产科,江苏南通226001

出　　处：《临床和实验医学杂志》2017年第23期2289-2293,共5页Journal of Clinical and Experimental Medicine

基　　金：南通市科技局重点病种的规范化临床诊疗研究(编号HS2014074);南通市卫计委青年基金(编号:WQ2014023;WQ2016030)

摘　　要：目的探讨亮丙瑞林和缩宫素对大鼠子宫内膜异位症模型的治疗作用及其对卵巢功能的影响。方法通过自体子宫移植方法建立大鼠腹壁子宫内膜异位症模型。将建模成功的SD大鼠随机分为实验组A(n=15),实验组B(n=15)和对照组C(n=15)。实验组A每天皮下注射亮丙瑞林20μg/kg,共28 d;实验组B每天肌内注射缩宫素160μg/kg,共28 d;对照组C每天皮下注射生理盐水0.1 ml,共28 d。末次给药24 h内,剖腹测量各组大鼠异位病灶的体积,应用RT-PCR检测大鼠异位病灶组织中血管内皮生长因子(VEGF)的表达量,称量大鼠右侧卵巢湿重,HE染色镜下观察右卵巢卵泡数量。结果 (1)治疗前,A、B两组大鼠异位病灶体积(43.39±11.95 mm^3、43.39±10.96 mm^3)与对照组C(42.52±6.14 mm^3)比较,差异无统计学意义(P>0.05)。治疗4周后,A、B组大鼠异位病灶体积(13.06±3.22mm^3、13.82±2.98 mm^3)较对照组C(39.31±7.45 mm^3)明显减小(P<0.05),A组较B组低,差异无统计学意义(P>0.05)。(2)治疗4周后,A、B组大鼠异位病灶组织中VEGF mRNA相对表达量(0.24±0.03、0.38±0.05)均较对照组C(0.96±0.01)明显下降(P<0.05);B组中VEGF mRNA表达量高于A组,差异有统计学意义(P<0.05)。(3)治疗后,A组大鼠卵巢湿重显著低于C组,差异有统计学意义(P<0.05)。A组大鼠卵巢中各级发育的卵泡数较C组明显减少,且闭锁卵泡数量增多,差异有统计学意义(P<0.05)。而B组大鼠卵巢湿重及卵泡数量较C组变化不明显。结论缩宫素与亮丙瑞林对大鼠子宫内膜异位症模型具有明显的治疗作用,下调VEGF的表达是其可能作用机制之一,缩宫素治疗子宫内膜异位症不影响大鼠卵巢的储备功能。ObjectiveTo discuss the therapeutic effects of oxytocin and leuprorelin on rat endometriosis model.MethodsThe rat model of abdominal wall endometriosis was established by autologous uterine transplantation.The SD rats were randomly divided into experimental group A(n=15),experimental group B(n=15)and control group C(n=15).The rats in group A were daily treated with leuprorelin20μg/kg by subcutaneous injection,a total of28days.The experimental group B received oxytocin160μg/kg by intramuscular injection every day,a total of28days.The control group C was daily given subcutaneous injection of saline0.1ml,a total of28days.After the end of treatment,the growth of ectopic lesions in each group was observed,the expression of vascular endothelial growth factor(VEGF)in ectopic endometrium tissue were detected by real-time polymerase chain reaction(RT-PCR),and the ovarian reserve including ovary weight and follicle number were observed.ResultsBefore treatment,ectopic lesion volume of group A,B(43.39±11.95mm3、43.39±10.96mm3)had no difference with the control group C(42.52±6.14mm3)(P>0.05).After4weeks of treatment,compared with the control group C(39.31±7.45mm3),ectopic lesion volume of group A,B(13.06±3.22mm3、13.82±2.98mm3)were decreased significantly(P<0.05),and group A was lower than that of group B(P>0.05).After4weeks of treatment,the expression of VEGF mRNA of rat ectopic lesion tissues in group A,B(0.24±0.03、0.38±0.05)were lower than those in the control group C(0.96±0.01)(P<0.05).The expression of VEGF mRNA of group B was slightly higher than that in group A(P<0.05).After treatment,the wet weight of ovary in group A was significantly lower than that of group C(P<0.05).The number of follicles in the ovary of group A was significantly lower than that in group C,while the number of locked follicles was increased(P<0.05).However,there was no significant change in the wet weight and the number of follicles in group C compared with those in group B.ConclusionLeuprolide and oxytocin have obvious therapeutic

关 键 词：大鼠 子宫内膜异位症模型 缩宫素 亮丙瑞林 血管内皮生长因子 卵巢储备功能 

分 类 号：R-332[医药卫生] R711.71