3种铁线莲挥发性成分的GC-MS分析  

Chemical Constituents of Essential Oil from Three Kinds of Clematis by GC-MS

作　　者：赵燕强[1,2,3] 王伟 杨立新[1] 杨叶昆[1,3] 周琳[1] 陈亚[1] 李忠荣[1] 高诚伟[3] 邱明华[1] ZHAO Yanqiang;WANG Wei;YANG Lixin;YANG Yekun;ZHOU Lin;CHEN Ya;LI Zhongrong;GAO Chengwei;QIU Minghua(Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204 China;Yunnan Forestry Technical College,Kunming 650224 China;College of Chemical Science and Engineering,Yunnan University,Kunming 650091 China)

机构地区：[1]中国科学院昆明植物研究所,云南昆明650204 [2]云南林业职业技术学院,云南昆明650224 [3]云南大学化学科学与工程学院,云南昆明650091

出　　处：《云南中医学院学报》2017年第5期85-91,共7页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81373288)

摘　　要：目的研究3种铁线莲的化学成分,为进一步开发利用3种铁线莲提供依据。方法采用气相色谱-质谱进行分析。结果对石油醚提取物进行了气相色谱-质谱分析,共鉴定出115个化合物。疏金毛铁线莲的主要成分是二十烷(8.45%),二十八烷(7.16%),角鲨烯(6.81%),二十七烷(5.78%),二十三烷(3.3%),二十四烷(2.38%)和BHT(1.11%);毛茛铁线莲的主要成分是二十八烷(30.37%),二十烷(18.45%),角鲨烯(3.06%),二十七烷(1.6%)和BHT(0.39%);合柄铁线莲的主要成分是二十烷(5.51%),十六酸(3.82%),亚油酸(3.44%),9,12-亚油酸甲酯(2.47%),亚油酸乙酯(1.56%),亚麻酸甲酯(1.32%),2-十二烷基醚乙醇(1.32%)和(Z)-9-烯十八酰胺(1.01%)。结论首次系统分析了3种铁线莲中挥发成分的组分和相对含量,为其进一步开发利用提供理论依据。其中角鲨烯是具有多种生物活性的长链多烯化合物,疏金毛铁线莲可作为富含角鲨烯的植物新资源。Objective To study the chemical constituents of three kinds of clematis and provide the basis for their furtherexploitation and utilization.Methods Chemical components were identified by GC-MS.Results The115volatile compounds wereisolated from the petroleum ether extracts of these plants and identified by GC-MS.The main composition of Clematis chrysocoma var.glabrescens was eicosane(8.45%),octacosane(7.16%),squalene(6.81%),heptacosane(5.78%),tricosane(3.3%),lignocerane(2.38%)and BHT(1.11%);The main composition of Clematis ranunculoide Franch was octacosane(30.37%),eicosane(18.45%),squalene(3.06%),heptacosane(1.6%)and BHT(0.39%);The main composition of Clematis connata DC was eicosane(5.51%),palmitic acid(3.82%),linoleic acid(3.44%),9,12-methyl linoleate(2.47%),ethyl linoleate(1.56%),methyl linolenate(1.32%),2-Lauryl ether ethanol(1.32%)and(Z)-9-Ene amide18(1.01%).Conclusion Systematic analysis was firstly conducted on the compositions and their relative contents of essential oils in three kinds of clematis,which would build foundation for further utilization of this species.Squalene is a variety of biological activity of long chain polyene compounds,thin Clematis chrysocoma var.glabrescens can be used as a new resource of plant squalene.

关 键 词：疏金毛铁线莲 毛茛铁线莲 合柄铁线莲 化学成分 气相色谱-质谱 

分 类 号：R284.1[医药卫生—中药学]