食用植物油中混入桐油的气相色谱-质谱法快速检测  

Rapid determination of tung oil in adulterated edible vegetable oil by gas chromatography-mass spectrometry

作　　者：蒲彦利[1] 张春玲[1] 王红波 曹杰[1] 和佳鸳 徐虹[1] PU Yan-li;ZHANG Chun-ling;WANG Hong-bo;CAO Jie;HE Jia-yuan;XU Hong(Zhenjiang Municipal Center for Disease Control and Prevention, Zhenjiang 212004, China)

机构地区：[1]江苏省镇江市疾病预防控制中心,镇江212004

出　　处：《江苏预防医学》2017年第6期630-632,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的建立食用植物油中桐油的气相色谱-质谱快速检测方法。方法样品经甲酯化处理后,HP-5MS毛细管色谱柱分离、质谱仪检测,以外标法定量。结果该检测方法,桐油在0.1%~5.0%浓度范围内呈较好的线性关系(r>0.99)。检出限可达0.01%,低、中、高加标浓度的平均回收率为95.5%、106.1%、112.1%,相对标准偏差(RSD)为7.0%、4.8%、4.4%。对2.0%桐油标准液重复测定6次,保留时间和定量离子峰面积的RSD分别为0.02%和1.07%。结论该方法前处理简单、快速,准确度和精密度较高,可用于突发性食物中毒时植物油中桐油的快速确证及定量测定。Objective To establish a rapid determination assay of tung oil in edible vegetable oil by gas chromatography-mass spectrometry(GC-MS).Methods The tung oil in sample was esterified and then separated by HP-5MS capillary column.GC-MS detector was used to analyze the concentration of tung oil.The external method was used for quantification.Results Good linear relationship was obtained in concentration range of0.1%-5.0%for tung oil,with the relative coefficient greater than0.99.The tung oil limit of detection was as low as0.01%.The average recoveries for spiked samples were95.5%,106.1%and112.1%for low,medium and high standard addition concentration,respectively,along with the relative standard deviations(RSDs)of7.0%,4.8%and4.4%,respectively.The standard2.0%tung oil sample analysis was repeated for6times,the relative standard deviation of retention time and quantitative ion peak area were0.02%and1.07%,respectively.Conclusion The established assay required simple pre-treatment and was quick with high accuracy and precision,which could be applied in qualitative and quantitative determination of tung oil in emergency food poisoning events.

关 键 词：植物油 桐油 气相色谱-质谱联用法 

分 类 号：R113[医药卫生—公共卫生与预防医学]