食管癌组织中CyclinB1/CDK1复合物表达量与血清及病灶中标志分子含量的相关性  

Correlation between the expression of CyclinB1/CDK1 complex in esophageal cancer tissue and the contents of marker molecules in serum and lesion

作　　者：季语祝[1] 陈曦[2] 谢刚[1] 谢春燕[1] 王芳[3] JI Yu-zhu;CHEN Xi;XIE Gang;XIE Chun-yan;WANG Fang(Department of Pathology, Mianyang Central Hospital in Sichuan Province, Mianyang City, Sichuan Province, 621000, China;Department of Clinical Laboratory, Mianyang Central Hospital in Sichuan Province, Mianyang City, Sichuan Province, 621000, China;Department of Pathology and Pathophysiology, Kunming Medical University in Yunnan Province, Kunming City, Yunnan Province, 650031, China)

机构地区：[1]四川省绵阳市中心医院病理科,四川绵阳621000 [2]四川省绵阳市中心医院检验科,四川绵阳621000 [3]云南省昆明医科大学病理学与病理生理学系,云南昆明650031

出　　处：《海南医学院学报》2017年第19期2716-2719,共4页Journal of Hainan Medical University

基　　金：云南省科技厅基础研究计划面上项目(2007C221M)~~

摘　　要：目的:研究食管癌组织中CyclinB1/CDK1复合物表达量与血清及病灶中标志分子含量的相关性。方法:选择在本院诊断为食管癌的患者作为食管癌组并采集食管癌病灶组织、癌旁病灶组织及血清标本,选择同期体检的健康志愿者作为对照组并采集血清标本。测定食管癌病灶组织、癌旁病灶组织中CyclinB1/CDK1复合物、抑癌基因、侵袭基因的表达量以及血清中肿瘤标志物的含量。结果:食管癌病灶中CyclinB1、CDK1、E2F-1的mRNA表达量显著高于癌旁病灶;食管癌组患者血清中肿瘤标志物CYFRA21-1、SCC-Ag、CD44v5、Stathmin的含量均显著高于对照组志愿者且与食管癌病灶中CyclinB1、CDK1、E2F-1的mRNA表达量呈正相关,食管癌病灶中PTEN、Spink8、p21、p18的mRNA表达量显著低于癌旁病灶且与食管癌病灶中CyclinB1、CDK1、E2F-1的mRNA表达量呈负相关,食管癌病灶中nestin、β-catenin、Snail、Vimentin的mRNA表达量显著高于癌旁病灶且与食管癌病灶中CyclinB1、CDK1、E2F-1的mRNA表达量呈正相关。结论:食管癌组织中CyclinB1/CDK1复合物的高表达能够促进癌细胞的增殖和侵袭。Objective:To study the correlation between the expression of CyclinB1/CDK1complex in esophageal cancer tissue and the contents of marker molecules in serum and lesion.Methods:Patients who were diagnosed with esophageal cancer in Mianyang Central Hospital between April2014and December2016were selected as esophageal cancer group,and the esophageal cancer lesion tissue,adjacent lesion tissue and serum samples were collected;healthy volunteers who received physical examination during the same period were selected as control group and serum samples were collected.The expression of CyclinB1/CDK1complex,tumor suppressor genes and invasion genes in esophageal cancer lesion tissue and adjacent lesion tissue as well as the levels of tumor markers in serum were detected.Results:CyclinB1,CDK1and E2F-1mRNA expression in esophageal cancer lesion were significantly higher than those in adjacent lesion;serum tumor markers CYFRA21-1,SCC-Ag,CD44v5and Stathmin levels of esophageal cancer group were significantly higher than those of control group and positively correlated with CyclinB1,CDK1and E2F-1mRNA expression in esophageal cancer lesion,PTEN,Spink8,p21and p18mRNA expression in esophageal cancer lesion were significantly lower than those in adjacent lesion and negatively correlated with CyclinB1,CDK1and E2F-1mRNA expression in esophageal cancer lesion,and nestin,β-catenin,Snail and Vimentin mRNA expression in esophageal cancer lesion were significantly higher than those in adjacent lesion and positively correlated with CyclinB1,CDK1and E2F-1mRNA expression in esophageal cancer lesion.Conclusion:The high expression of CyclinB1/CDK1complex in esophageal cancer tissue can promote the proliferation and invasion of cancer cells.

关 键 词：食管癌 细胞周期蛋白B1 细胞周期蛋白依赖性激酶1 肿瘤标志物 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]