仁用杏果肉3个发育阶段的转录组分析  

Transcriptome Analysis of Kernel-apricot Sarcocarps during Three Development Stages

作　　者：谭金花 候丽秀 刘永红[1] 王胜琪[1] 魏安智[1] 刘玉林[1] Tan Jinhua;Hou Lixiu;Liu Yonghong;Wang Shengqi;Wei Anzhi;Liu Yulin(College of Forestry, Northwest A&F University, Yangling,Shaanxi 712100, China)

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北植物学报》2017年第10期2017-2024,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金："十二五"国家科技支撑计划(2013BAD14B02);中央高校基本科研业务费专项资金(2452015167)

摘　　要：仁用杏果肉糖酸比低是造成其鲜食性差的主要原因。该研究以仁用杏‘山苦2号’开花后30、60和90d的果肉为材料,利用RNA-seq对仁用杏果肉进行转录组测序及差异表达基因的筛选,以了解可能与糖酸合成相关的基因及其表达模式,为进一步解析仁用杏果肉糖酸比低的分子调控模式与机制,深入研究仁用杏果肉糖酸代谢转化机制提供依据。结果显示:(1)在仁用杏果肉发育的3个阶段共获得差异表达的单基因簇(unigene)28 989条;根据单基因簇的功能注释,共发掘出19条单基因簇编码5个与仁用杏果肉中糖合成代谢相关的酶,54条单基因簇编码15个与仁用杏果肉中酸合成代谢相关的酶。(2)在所选3个发育阶段的仁用杏果肉中共发现9条与糖代谢相关的单基因簇和27条与酸代谢相关的单基因簇呈现差异表达。(3)编码与蔗糖合成积累相关的蔗糖磷酸酶和蔗糖合成酶的单基因簇主要呈下调表达,而与糖分解相关的果糖激酶和己糖激酶却呈上调表达。(4)与仁用杏果肉中主要有机酸组分苹果酸积累相关的苹果酸脱氢酶主要呈上调表达,而分解苹果酸的苹果酸酶呈下调表达。研究推测,仁用杏果肉在发育中糖积累受阻,而有机酸却得到大量的合成积累,这可能是造成仁用杏果肉糖酸比例小,鲜食口感差的主要内在因素之一。The low ratio of sugars and organic acids is one of the major reasons for poor taste in kernel-apri-cot sarcocarp.In this study,to understand the expression patterns of genes which may be associated with sugar and organic acids synthesis and to indentify the molecular regulation pattern and mechanism of the low ratio of sugars and organic acids,we used RNA seq technique to obtain the transcriptomes and expression profiles of three developing stages(30,60and90days after flowering)about kernel apricot sarcocarp of‘Shanku2hao’to provide a basis for the study of the metabolic and conversion mechanism of sugars and organic acids in kernel apricot sarcocarp.The results showed that:(1)28989differently expressed unigenes were obtained during the three developing stages.According to the unigene annotation,5enzymes encoded by19unigenes related to sugar biosynthesis and metabolism and15enzymes encoded by54unigenes involved in organic acids biosynthesis and metabolism were obtained;(2)Among the differently expressed unigenes,9and27unigenes of them which related to sugar and organic acid biosynthesis and metabolism were differently expressed in the three developing stages,respectively;(3)Most of the unigenes encoding the sucrose phosphate synthase and sucrose synthase which related to the sugar accumulation were down regulated,but the fructokinase and hexokinase that involved in sugar catabolism were up regulated;(4)The unigenes encoding malate dehydrogenase which associated with accumulation of malic acid was the main components of organic acids in kernel apricot sarcocarp were mainly up regulated.However,the malic enzyme catalyzed the breakdown of malic acid was down regulated.It can be sure that the accumulation of sugars was restrained,but the organic acids were synthesized and accumulated in large quantities during the developments of kernel apricot sarcocarp.This may be one of the main internal factors that contribute to the low ratio of sugars and organic acids and the poor taste of kernel apricot sarcocarp.

关 键 词：仁用杏 果肉 转录组 糖酸代谢 

分 类 号：Q789[生物学—分子生物学] S662.9[农业科学—果树学]