党参地上部分降血脂活性成分的分离鉴定  

Isolation and Identification of Antiobesity Ingredients in Aerial Part of Codonopsis pilosula (Franch.) Nannf

作　　者：谢敏 李秀壮[1] 刘景坤 燕志强[1] 宿安祥 秦波[1] Xie Min;Li Xiuzhuang;Liu Jingkun;Yan Zhiqiang;Su Anxiang;Qin Bo(CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou 730000, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute for the Control of Agrochemicals, Drug Information Department, Beijing 100125, China)

机构地区：[1]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,兰州730000 [2]中国科学院大学,北京100049 [3]农业部农药检定所药情信息处,北京100125

出　　处：《西北植物学报》2017年第10期2082-2086,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31570354;21302195;31300290);中国科学院"西部之光"人才培养计划;甘肃省省青年科技基金计划(1506RJYA294);中国科学院兰州分院甘肃省院地合作项目

摘　　要：该研究以党参的茎叶为材料,采用硅胶柱层析、MCI柱层析、制备薄层等方法,分离化合物并鉴定,以全面了解党参的化学成分,明确其药效成分及活性作用。结果显示:(1)从党参的茎叶中共分离得到了8个化合物,其中包括2个甾体、2个脂肪酸、1个甘油酯、3个甘油糖酯,具体化合物为:α-菠甾醇(1)、(22E)-5α,8α-桥二氧麦角甾-6,22-二烯-3β-醇(2)、亚油酸(3)、1-亚油酸甘油酯(4)、硬脂酸(5)、3-α-亚麻酸甘油酯1-O-[α-D-半乳糖基-(1→6)-O-β-D-半乳糖苷](6)、3-α-亚麻酸甘油酯1-O-β-D-半乳糖苷和3-(7,10,13-十六碳三烯酸)甘油酯1-O-β-D-半乳糖苷混合物(7),其中7为两种化合物混合存在,难以分离。(2)化合物2、4、6、混合物7均为首次从党参中分离得到。(3)化合物1、2、3、6及混合物7均对胰脂肪酶活性具有一定的抑制作用;由于胰脂肪酶在脂质的代谢中起着重要作用,推测化合物1、2、3、6及混合物7具有抑制脂肪积累的作用,为党参中降血脂的活性成分。Chemical constitution was thoroughly investigated to explicit active ingredients and action mode through isolation and identification of compounds using silica gel column chromatographic,MCI gel column chromatographic and preparative thin layer chromatography method with aerial part of Codonopsis pilosula(Franch.)Nannf as source material.Results obtained as follows:(1)eight compounds were isolated from aerial part of Codonopsis pilosula including two steroids,two fatty acids,one glyceride,and three glycolipids:αspinasterol(1),(22E)-5α,8α-epidioxy ergosta-6,22-dien-3β-ol(2),9,12-linoleic acid(3),1-α-linoleoylglycerol(4),stearic acid(5),3-α-linolenoylglycerol1-O-[α-D-galactopyranosyl-(1→6)O-β-D-galactopyranoside(6),3-α-linolenoylglycerol1-O-β-D galactopyranoside and3-(7,10,13-hexadecatrienoylglycerol)1-O-β-D galactopyranoside mixture(7).(2)Compounds2,4,6and7were firstly isolated from C.pilosula.(3)Compounds1,2,3,6and complex7could inhibit activities of pancreatic lipase.As the pancreatic lipase was responsible for metabolism of lipid,compounds1,2,3,6and complex7probably would reduce lipid accumulation and were defined as anti obesity ingredients of C.pilosula.

关 键 词：党参 降血脂 甘油糖酯 不饱和脂肪酸 

分 类 号：Q501[生物学—生物化学] Q946.8