新喜树碱衍生物的核磁共振谱分析与结构鉴定  

Structure Identification of New Camptothecin Derivatives by Nuclear Magnetic Resonance Spectrometry

作　　者：高旭东[1] 赵晓博 胡玥[1] 火婷 邵士俊[1] 师彦平[1] GAO Xu-dong;ZHAO Xiao-bo;HU Yue;HUO Ting;SHAO Shi-jun;SHI Yan-ping(CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics;Chinese Academy of Sciences(CAS), Lanzhou 730000, China)

机构地区：[1]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室,甘肃兰州730000

出　　处：《分析测试技术与仪器》2017年第4期208-213,共6页Analysis and Testing Technology and Instruments

基　　金：中国科学院仪器设备功能开发技术创新项目(项目编号:lg201509)资助

摘　　要：以20(S)-喜树碱(CPT)为起始原料,对其进行结构修饰,合成了一种新的喜树碱衍生物CPT-A.并利用1D(~1H、^(13)C和DEPT135)和2D(异核单量子相关谱和异核多碳相关谱)核磁共振(NMR)技术对该化合物进行了结构确定,详细归属了CPT-A^1H和^(13)C NMR谱的化学位移,喜树碱骨架的指认结果与文献报道基本一致,并发现喜树碱骨架6位碳与取代基苯环3’位碳的化学位移相同,谱峰完全重叠,这在^(13)C谱中是不常见的.研究结果可为喜树碱类天然产物的发现和结构鉴定提供NMR数据支持和方法指引.A new camptothecin(CPT)derivative CPT-A was synthesized by modifying camptothecin,one of the most important natural products.Its chemical structure was identified using1D and2D nuclear magnetic resonance(NMR)techniques.Both1H and13C chemical shifts of CPT-A were exactly assigned.The results of the identification of camptothecin skeleton are basically consistent with those reported in the literature.In this study,the number6carbon of camptothecin skeleton and the number3’carbon of benzene are completely overlapped.This phenomenon is seldom seen in the13C NMR spectra.NMR data support for the discovery and structural identification of natural products of camptothecin derivatives can be obtained in the study.Furthermore,a methodology was involved in the analysis of NMR spectra.

关 键 词：喜树碱衍生物 CPT-A 核磁共振 谱图解析 结构鉴定 

分 类 号：O657.3[理学—分析化学]