Effect of Tb/Mg doping on composition and physical properties of hydroxyapatite nanoparticles for gene vector application  

Tb/Mg掺杂对羟基磷灰石纳米颗粒基因载体理化特性的影响（英文）

作　　者：Liang-jian CHEN Tian CHEN Jun CAO Bei-lei LIU Chun-sheng SHAO Ke-chao ZHOU Dou ZHANG 陈良建;陈恬;曹君;刘蓓蕾;邵春生;周科朝;张斗(中南大学湘雅三医院口腔科,长沙410013;中南大学粉末冶金重点实验室,长沙410083;中南大学湘雅医学院,长沙410013)

机构地区：[1]Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha 410013, China [2]State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China [3]Xiangya School of Medicine, Central South University, Changsha 410013, China

出　　处：《Transactions of Nonferrous Metals Society of China》2018年第1期125-136,共12页中国有色金属学报（英文版）

基　　金：Project(2015WK3012) supported by the Hunan Provincial Science and Technology Department Project,China;Project(81571021) supported by the National Natural Science Foundation of China;Project(225) supported by the High Level Health Personnel in Hunan Province,China;Project(621020094) supported by the State Key Laboratory of Powder Metallurgy of Central South University,China;Project(20160301) supported by New Talent Project of the Third Xiangya Hospital of Central South University,China

摘　　要：Transfection efficiency of hydroxyapatite nanoparticles(HAnps)is relative to the particle size,morphology,surface charge,surface modifier and so on.This study prepared HAnps with doped Tb/Mg by hydrothermal synthesis method(HTSM)and investigated the effects of different Tb/Mg contents on the morphology,particle size,surface charge,composition and cellular endocytosis of HAnps.The results showed that Mg-HAnps possessed better dispersion ability than Tb-HAnps.With increasing doping content of Tb/Mg-HAnps,the granularity of Tb-HAnps increased,while that of Mg-HAnps declined.Both particle size and zeta potential of Mg-HAnps were lower than those of Tb-HAnps.7.5%Mg-doping HAnps presented relatively uniform slender rod morphology with average size of30nm,while10%Mg-doping HAnps were prone to agglomeration.Moreover,Mg-HAnps-GFP(green fluorescent protein)endocytosed by MG63cells was dotted in the perinuclear region,while Tb-HAnps were more likely to aggregate.In conclusion,as gene vectors,Mg-HAnps showed enhanced properties compared to Tb-HAnps.羟基磷灰石纳米颗粒(HAnps)基因载体的转染效率与粒径、形貌、表面电荷、表面改性等有关。本研究通过水热合成法制备掺杂Tb/Mg的HAnps,观察Tb/Mg掺杂量对HAnps形貌、粒径、表面电荷、成分和细胞胞吞作用的影响。结果表明,掺Mg组的分散性优于掺Tb组的分散性。增加掺杂量使Tb-HAnps的粒径增大,而Mg-HAnps的粒径减小。掺Mg组的粒径及zeta电位均低于掺Tb组的。掺Mg量为7.5%的HAnps平均粒径约30 nm,呈相对均匀的细长杆状,而掺Mg量为10%的HAnps易于团聚。被胞吞入MG63细胞的Mg-HAnps-GFP呈点状分布于核周区域并形成荧光圈,而Tb-HAnps易于团聚。因此,作为基因载体,Mg-HAnps明显优于Tb-HAnps。

关 键 词：hydroxyapatite nanoparticles gene vector ENDOCYTOSIS DOPING fluorescence labeling 

分 类 号：TB383.1[一般工业技术—材料科学与工程]