Electrooxidation of sulfanilamide and its voltammetric determination in pharmaceutical formulation,human urine and serum on glassy carbon electrode  

作　　者：Bruno R.L.Ferraz Tiago Guimaraes Demetrius Profeti Luciene P.R.Profeti 

机构地区：[1]Departamento de Biologia,Universidade Federal do Espírito Santo,Alegre 29.500-000,ES,Brazil [2]Departamento de Química e Física,Universidade Federal do Espírito Santo,Alegre 29.500-000,ES,Brazil

出　　处：《Journal of Pharmaceutical Analysis》2018年第1期55-59,共5页药物分析学报（英文版）

基　　金：UFES;CNPq,CAPES(23068719374/2017-70);FAPES(54693900/2011,54694442/2011,60125730/2012,53671880/2011)for the financial support

摘　　要：For the first time, sulfanilamide(SFD) was determined in otologic solution, human urine and serum by electroanalytical techniques on glassy carbon electrode(GCE). The cyclic voltammetry(CV) experiments showed an irreversible oxidation peak at t 1.06 V in 0.1 mol/L BRBS(p H ? 2.0) at 50 m V/s. Different voltammetric scan rates(from 10 to 250 m V/s) suggested that the oxidation of SFD on the GCE was a diffusioncontrolled process. Square-wave voltammetry(SWV) method under optimized conditions showed a linear response to SFD from 5.0 to 74.7 μmol/L(R ? 0.999) with detection and quantification limits of 0.92 and3.10 μmol/L, respectively. The developed SWV method showed better results for detection limit and linear range than the chronoamperometry method. It has been successfully applied to determine SFD concentration in pharmaceutical formulation, human urine and serum samples with recovery close to 100%.For the first time, sulfanilamide(SFD) was determined in otologic solution, human urine and serum by electroanalytical techniques on glassy carbon electrode(GCE). The cyclic voltammetry(CV) experiments showed an irreversible oxidation peak at t 1.06 V in 0.1 mol/L BRBS(p H ? 2.0) at 50 m V/s. Different voltammetric scan rates(from 10 to 250 m V/s) suggested that the oxidation of SFD on the GCE was a diffusioncontrolled process. Square-wave voltammetry(SWV) method under optimized conditions showed a linear response to SFD from 5.0 to 74.7 μmol/L(R ? 0.999) with detection and quantification limits of 0.92 and3.10 μmol/L, respectively. The developed SWV method showed better results for detection limit and linear range than the chronoamperometry method. It has been successfully applied to determine SFD concentration in pharmaceutical formulation, human urine and serum samples with recovery close to 100%.

关 键 词：SQUARE-WAVE VOLTAMMETRY SULFANILAMIDE GLASSY carbon electrode Biological FLUIDS 

分 类 号：R[医药卫生]