Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry  

作　　者：Yorick Janssens Frederick Verbeke Nathan Debunne Evelien Wynendaele Kathelijne Peremans Bart De Spiegeleer 

机构地区：[1]Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University [2]Department of Veterinary Medical Imaging and Small Animal Orthopaedics, Faculty of Veterinary Medicine, Ghent University

出　　处：《Journal of Pharmaceutical Analysis》2018年第1期69-74,共6页药物分析学报（英文版）

基　　金：the‘Research Foundation–Flanders(FWO)’(Grant no.1S21017N to Nathan Debunne);the‘Institute for the Promotion of Innovation through Science and Technology in Flanders(IWT-Vlaanderen)’(Grant no.131356 to Frederick Verbeke)

摘　　要：Five different quorum sensing peptides(QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1%(m/v) formic acid as mobile phase. Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current(TIC) spectra. Non-iodinated peptides and mono-and diiodinated peptides(NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low(2%) to high(57%).Five different quorum sensing peptides(QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1%(m/v) formic acid as mobile phase. Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current(TIC) spectra. Non-iodinated peptides and mono-and diiodinated peptides(NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low(2%) to high(57%).

关 键 词：Peptide IODINATION LC–UV/MS QUORUM sensing Bolton-Hunter 

分 类 号：R[医药卫生]