弥漫大B细胞淋巴瘤内TNFAIP3、PDE4B对肿瘤细胞凋亡、侵袭的影响  

Effects of TNFAIP3 and PDE4B on apoptosis and invasion of tumor cells in diffuse large B cell lymphoma

作　　者：吴必嘉[1] 龚峻梅[1] 陈娟娟[1] 黄斯勇[2] 高飞 WU Bi-jia;GONG Jun-mei;CHEN Juan-juan;HUANG Si-yong;GAO Fei(Department of Tumor Hematology, the 421 Hospital of Chinese People's Liberation Army, Guangzhou, Guangdong Province, 510318;Department of Hematology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi Province, 710038;Guangzhou PKGENE Science and Technology Co., Ltd., Guangzhou, Guangdong Province, 510045)

机构地区：[1]中国人民解放军第四二一医院肿瘤血液科,广东广州510318 [2]第四军医大学唐都医院血液科,陕西西安710038 [3]广州普康基因科技有限公司,广东广州510045

出　　处：《海南医学院学报》2017年第24期3422-3425,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81300397)~~

摘　　要：目的:研究弥漫大B细胞淋巴瘤(DLBCL)内肿瘤坏死因子诱导蛋白3基因(TNFAIP3)、磷酸二酯酶4B(PDE4B)对肿瘤细胞凋亡、侵袭的影响。方法:选择2014年8月~2017年7月期间在广州四二一医院诊断为弥漫大B细胞淋巴瘤的68例患者作为研究的DLBCL组,选择同期因淋巴结肿大在广州四二一医院接受淋巴结穿刺活检且诊断为反应性淋巴结增生的34例患者作为对照组。测定淋巴结组织中TNFAIP3、PDE4B以及凋亡基因、侵袭基因的表达量。结果:DLBCL组病灶组织中TNFAIP3、PTEN、PTPL1、Bax的蛋白表达量显著低于对照组(P<0.05),PDE4B、c-myc、AURKA、AURKB、PLK1、Ets-1、β-catenin、MMP7、MMP9、CD44的蛋白表达量显著高于对照组(P<0.05);DLBCL病灶组织中PTEN、PTPL1、Bax的蛋白表达与TNFAIP3的蛋白表达量呈正相关,与PDE4B的蛋白表达量呈负相关;c-myc、AURKA、AURKB、PLK1、Ets-1、β-catenin、MMP7、MMP9、CD44的蛋白表达量与TNFAIP3的蛋白表达量呈负相关,与PDE4B的蛋白表达量呈正相关。结论:弥漫大B细胞淋巴瘤内TNFAIP3的低表达以及PDE4B的高表达能够拮抗肿瘤细胞凋亡、促进肿瘤细胞侵袭。Objective:To study the effects of TNFAIP3and PDE4B on apoptosis and invasion of tumor cells in diffuse large B cell lymphoma.Methods:68patients who were diagnosed with diffuse large B cell lymphoma in Guangzhou421Hospital of PLA between August2014and July2017were selected as the DLBCL group of the study,and34patients who accepted lymph node biopsy due to lymphadenectasis and were diagnosed with reactive lymphoid hyperplasias in Guangzhou421Hospital of PLA during the same period were selected as the control group.The expression levels of TNFAIP3,PDE4B,apoptosis genes and invasion genes in lymph node tissue were determined.Results:TNFAIP3,PTEN,PTPL1and Bax protein expression in lesion tissue of DLBCL group were significantly lower than those of control group whereas PDE4B,c-myc,AURKA,AURKB,PLK1,Ets-1,β-catenin,MMP7,MMP9and CD44protein expression were significantly higher than those of control group;PTEN,PTPL1and Bax protein expression in DLBCL lesions were positively correlated with TNFAIP3protein expression and negatively correlated with PDE4B protein expression;c-myc,AURKA,AURKB,PLK1,Ets-1,β-catenin,MMP7,MMP9and CD44protein expression were negatively correlated with TNFAIP3protein expression and positively correlated with PDE4B protein expression.Conclusion:The low expression of TNFAIP3and the high expression of PDE4B in diffuse large B cell lymphoma can antagonize tumor cell apoptosis and promote tumor cell invasion.

关 键 词：弥漫大B细胞淋巴瘤(DLBCL) 肿瘤坏死因子诱导蛋白3基因(TNFAIP3) 磷酸二酯酶4B(PDE4B) 凋亡 侵袭 

分 类 号：R733.4[医药卫生—肿瘤]