调强放疗+局部热疗对肝癌病灶内癌细胞凋亡及侵袭的影响  

The effects of intensity modulated radiation therapy+local hyperthermia on the cancer cell apoptosis and invasion in liver cancer lesion

作　　者：苏文兵[1] 杨丽 兰文权 SU Wen-bing;YANG Li;LAN Wen-quan(Digestive System Department, Xiantao First People's Hospital in Hubei Province, Xiantao, Hubei Province, 433000;Hepatobiliary Surgery Department, Xiantao First People's Hospital in Hubei Province, Xiantao, Hubei Province, 433000)

机构地区：[1]湖北省仙桃市第一人民医院消化内科,湖北仙桃433000 [2]湖北省仙桃市第一人民医院肝胆外科,湖北仙桃433000

出　　处：《海南医学院学报》2017年第24期3429-3432,共4页Journal of Hainan Medical University

基　　金：仙桃市科学技术局2013年指导性科技研究与开发计划项目(2013-1)~~

摘　　要：目的:探讨调强放疗+局部热疗对肝癌病灶内癌细胞凋亡及侵袭的影响。方法:选择2015年11月~2017年2月间在本院确诊并接受治疗的中晚期原发性肝癌患者94例,经随机数表法分为对照组、实验组,各47例。对照组患者接受调强放疗,实验组患者接受调强放疗+局部热疗。治疗前后对两组病灶进行腹腔穿刺活检,采用荧光定量PCR法检测标本组织中凋亡、侵袭基因的表达量。结果:治疗前,两组患者病灶组织中凋亡、侵袭基因表达量的差异无统计学意义。治疗后,实验组病灶组织中凋亡基因Fas、caspase-3、Bax、p53mRNA的表达量高于对照组,FasL、Bcl-2 mRNA的表达量低于对照组;实验组病灶组织中侵袭基因Cofilin-1、Bmi-1、STAT3、SOX18 mRNA的表达量低于对照组,Tip30、TP53IP1mRNA的表达量高于对照组。结论:中晚期原发性肝癌患者接受调强放疗+局部热疗,可有效促进癌细胞凋亡并抑制其侵袭活性。Objective:To investigate the effects of intensity modulated radiation therapy+local hyperthermia on the cancer cell apoptosis and invasion in liver cancer lesion.Methods:A total of94patients with middle-advanced primary liver cancer who were diagnosed and treated in this hospital between November2015and February2017were divided into control group(n=47)and experimental group(n=47)by random number table method.Control group received intensity modulated radiation therapy and experimental group received intensity modulated radiation therapy+local hyperthermia.Both groups accepted peritoneal lesion biopsy before and after treatment,and the expression of apoptosis and invasion genes in specimen tissue were detected by fluorescence quantitative PCR.Results:There was no statistically significant difference in apoptosis and invasion gene expression between the two groups of patients before treatment.After treatment,apoptosis genes Fas,caspase-3,Bax and p53mRNA expression in lesion tissue of experimental group were higher than those of control group whereas FasL and Bcl-2mRNA expression were lower than those of control group;invasion genes Cofilin-1,Bmi-1,STAT3and SOX18mRNA expression in lesion tissue of experimental group were lower than those of control group whereas Tip30and TP53IP1mRNA expression were higher than those of control group.Conclusion:intensity modulated radiation therapy+local hyperthermia can effectively promote cancer cell apoptosis and inhibit its invasion activity in patients with middle and advanced primary liver cancer.

关 键 词：调强放疗+局部热疗对肝癌病灶内癌细胞凋亡及侵袭的影响 

分 类 号：R735.7[医药卫生—肿瘤]