全基因组关联定位籼稻种质资源外观和加工品质QTL  

Genome-wide Association Study of Grain Appearance and Milling Quality in a Worldwide Collection of Indica Rice Germplasm

作　　者：方雅洁 朱亚军 吴志超[2] 陈凯 申聪聪 石英尧[1] 徐建龙[2,3,4] FANG Ya-Jie;ZHU Ya-Jun;WU Zhi-Chao;CHEN Kai;SHEN Cong-Cong;SHI Ying-Yao;XU Jian-Long(Anhui Agricultural University,Hefei230036,Anhui,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing100081,China;Agricultural Genomics Institute,Chinese Academy of Agricultural Sciences,Shenzhen518210,Guangdong,China;Shenzhen Institute of Breeding & Innovation,Chinese Academy of Agricultural Sciences,Shenzhen518120,Guangdong,China)

机构地区：[1]安徽农业大学,安徽合肥230036 [2]中国农业科学院作物科学研究所,北京100081 [3]中国农业科学院农业基因组研究所,广东深圳518210 [4]中国农业科学院深圳生物育种创新研究院,广东深圳518210

出　　处：《作物学报》2018年第1期32-42,共11页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2014AA10A601);农业部引进国际先进农业科学技术(948)项目(2016-X16);深圳孔雀团队计划(20130415095710361);中国农业科学院科技创新工程团队项目资助~~

摘　　要：以400份籼稻变异丰富的种质资源材料在2个环境下考察外观与加工品质性状表型,利用404k高密度SNP基因型进行全基因组关联分析,挖掘影响外观与加工品质性状的重要位点。结果表明,粒形和籼米的外观和加工品质密切相关,垩白性状降低稻米的精米率和整精米率,除粒形外,籼稻的外观和加工品质明显受到环境影响。全基因组关联分析共鉴定到39个QTL,其中17个为新位点,6个新位点(q MRR9、q GL2、q GL10、q GW1、q GLWR1和q GLWR2.1)在2个环境下均被检测到,暗示这6个位点在水稻外观与加工品质上可能是不依赖于环境稳定表达的QTL。此外,21个控制稻米外观品质的位点很可能具一因多效。结合前人研究结果,我们推论q SW5、GSE5和GS3在籼稻外观和加工品质性状上扮演着关键性角色。研究结果为克隆新的外观与加工品质基因,以及通过分子手段加速培育优质高产籼稻新品种提供了指导信息。Appearance and milling quality are two crucial properties of rice affecting their market acceptability.Understanding the genetic basis of rice grain quality could improve the efficiency of breeding for high quality.Here we carried out a genome-wide association analysis using the404k SNP genotype and data of grain appearance and milling quality for a diverse panel of400indica accessions selected from3k Rice Genome Project collected in two environments.These were closely correlation between grain shape and quality(appearance and milling)of indica rice.The chalk trait reduced milled rice rate and high milled rice rate.In addition to grain shape,the phenotype value of appearance and milling quality traits was obviously affected by environments.Total of39QTL were detected which was significantly associated with grain appearance and milling quality in two environments and17of them were new QTL.Six new QTL(qMRR9,qGL2,qGL10,qGW1,qGLWR1,and qGLWR2.1)with minor or small effect were considered as stably expressed QTL because they were simultaneously identified in two environments.In addition,we found21QTL probably having multiple-effect in appearance and milling quality of indica rice.Combined with the results of previous studies,we concluded that qSW5,GSE5,and GS3play an important role in affecting appearance and milling quality.This research provides valuable information for cloning QTL controlling appearance and milling quality and breeding rice with high yield and good quality by molecular techniques.

关 键 词：籼稻种质资源 外观与加工品质 全基因组关联分析 数量性状座位 

分 类 号：S511.21[农业科学—作物学]