津田芜菁BrSIZ1基因克隆、定位及表达  

Cloning, Location, and Expression of BrSIZ1 in Brassica rapa L. subsp. rapifera ‘Tsuda'

作　　者：罗云[1] 马璇[1] 谷俊辰 闫海芳[1] LUO Yun;MA Xuan;GU Jun-Chen;YAN Hai-Fang(College of Life Sciences,Northeast Forestry University,Harbin150040,Heilongjiang,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《作物学报》2018年第1期75-81,共7页Acta Agronomica Sinica

基　　金：中央高校基本科研业务费专项(DL12CA10);东北林业大学科研训练项目(KY2015053)资助;supported by the Fundamental Research Funds for the Central Universities(DL12CA10);the Research Training Program of Northeast Forestry University(KY2015053)

摘　　要：SIZ1是植物细胞蛋白质翻译后修饰SUMO化的E3连接酶,参与植物蛋白相互作用、定位和抗逆反应。为研究BrSIZ1在津田芜菁中的表达特性,本研究克隆了津田芜菁SIZ1基因全长c DNA序列,命名为BrSIZ1(Gen Bank登录号为KY441465),该基因全长2754 bp,其ORF全长2571 bp,编码856个氨基酸残基的多肽。构建了BrSIZ1-GFP表达载体进行亚细胞定位研究,结果显示BrSIZ1-GFP定位于细胞核内,可能在细胞核中发挥其功能。利用荧光定量PCR检测表明,该基因表达量在叶片中最高,在幼苗和红色根皮中次之,表达具有组织特异性。而且BrSIZ1在芜菁根皮中的表达受长波紫外线(UV-A)诱导,在4°C、37°C胁迫的幼苗中,表达量增加。SIZ1,a SUMO E3ligase involved in post-translation of proteins in plant cells,plays a role in protein interaction,location,and response to environmental stresses.In order to elucidate the expression profile of SIZ1in Tsuda,cDNA of SIZ1gene was isolated from Tsuda.This gene was named BrSIZ1(GenBank accession number KY441465).BrSIZ1was2754bp in fulll ength cDNA and2571bp in full length open reading frame(ORF),encoding a peptide with856amino acids.A BrSIZ1-GFP expression vector was constructed to analysis the subcellular localization.BrSIZ1-GFP was localized to nucleus,indicating that BrSIZ1may play an important role in the nucleus.Quantitative-PCR analysis showed that the BrSIZ1was expressed meetly in leaf and secondly in young seedling and red root epidermis,showing tissue specificity.The expression of the BrSIZ1was induced by UV-A light in the root epidermis.The transcript level of BrSIZ1was up-regulated when treated with temperature of4°C or37°C in young seedling.

关 键 词：津田芜菁 SIZ1 基因克隆 亚细胞定位 表达分析 

分 类 号：S631.3[农业科学—蔬菜学]