穴位埋线对实验自身免疫性葡萄膜炎大鼠眼部炎症影响的研究  

Effect of acupoint catgut embedding on ocular inflammation in experimental autoimmune uveitis rats

作　　者：赵建英[1] 郝小波[1] 郑凤鸣 黄素珍 吴含青 郭大东[5] ZHAO Jianying;HAO Xiaobo;ZHENG Fengming;HUANG Suzhen;WU Hanqing;GUO Dadong(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning,Guangxi,530023;Chinese Medicine Hospital Shangyu Shaoxing,Shaoxing,Zhejiang,312300;Dongguan Humen Zhong Yiyuan,Dongguan,Guangdong,523902;Guangxi International Zhuang Hospital,Nanning,Guangxi,530001;Eye Institute of Shandong University of TCM,Jinan,Shandong,250002)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]绍兴市上虞中医医院,浙江绍兴312300 [3]东莞市虎门中医院,广东东莞523902 [4]广西国际壮医医院,广西南宁530001 [5]山东中医药大学眼科研究所,山东济南250002

出　　处：《中医眼耳鼻喉杂志》2017年第4期194-196,共3页Journal of Chinese Ophthalmology and Otorhinolaryngology

基　　金：1广西卫生自筹计划课题(Z20170279)2.2017广西中医药大学校级面上项目(2017MS029)3.国家自然科学基金(81360558);4.广西自然科学基金项目(2010GXNSFA013208);5.广西自然科学基金项目(0342042)6.广西科技攻关计划项目(12300020)7.广西高校科学技术研究项目(KY2015YB157)8.山东省中医药科技发展计划项目(编号:2013ZDZK-083)

摘　　要：目的探讨穴位埋线疗法对治疗EAU大鼠眼部炎症情况的影响变化。方法Lewis大鼠随机分为对照组3只、EAU组24只和穴位埋线(ACE)组24只。除对照组外,均使用光感受器间维生素A结合蛋白(IRBP)免疫造模形成EAU组,ACE组为造模成功后次日进行穴位埋线,分别观察眼前段炎症变化并记录免疫后9d、13d、18d及23d不同时间段的眼部房水蛋白量。结果造模后13d是炎症的高峰期,该时段模型鼠眼前段炎症及房水蛋白量均达到高峰,EAU组房水蛋白浓度为41.15±3.91mg/ml,ACE组13d房水蛋白浓度为21.85±1.70mg/m;不仅如此,在观察的4个时间段中,ACE组房水蛋白量均低于EAU组(P<0.01)。结论穴位埋线疗法能减轻EAU大鼠眼前段炎症反应,同时还可以减轻由于眼前段炎症导致的房水蛋白量的急剧升高,因此该疗法在调节免疫状态方面是一种有积极有效的绿色疗法。ObjectiveTo explore the effect of acupoint catgut embedding therapy on ocular inflammation in EAU rats.MethodsLewis rats were randomly divided into control group(n=3),EAU group(n=24)and acupoint catgut embedding(ACE)group(n=24).(IRBP)was used to form EAU group.The ACE group was treated with acupoint catgutation after the success of the model.The changes of the anterior segmental inflammation were observed and9days after the immunization.13d,18d and23d at different times of eye aqueous protein.ResultsT The peak of inflammation and the amount of aqueous protein in the model group were41.15±3.91mg/ml,and the aqueous protein concentration in the ACE group was21.85±1.70mg/m;not only that,in the observation of the four time periods,ACE group of aqueous protein levels were lower than EAU group(P<0.01).ConclusionAcupoint catgut embedding therapy can reduce the anterior segment of the EAU rat inflammatory response,but also can reduce the anterior segment of the inflammation caused by the rapid increase in the amount of aqueous humor protein,so the regulation of immune status is a positive and effective green therapy.

关 键 词：穴位埋线 自身免疫性葡萄膜炎 实验 大鼠 房水蛋白 

分 类 号：R77[医药卫生—眼科]