补精益视片对SD大鼠慢性高眼压模型外侧膝状体脑源性神经营养因子表达的影响  

Effects of BuJingYiShi tablets on expression of brain derived neurotrophin factor in lateral geniculate in rat model of chronic elevated intraocular pressure

作　　者：李翔[1] 柯欣怡 刘红佶 袁铭悦 李祥玉 李华宏 王泰 田梦瑶 杨凤娇 LI Xiang;KE Xinyi;LIU Hongji;YUAN Mingyue;Li Xiangyu;LI Huahong;WANG Tai;TIAN Mengyao;YANG Fengjiao(Department of Ophthalmology,The Teaching Hospital of Chengdu University of TCM,Chengdu,Sichuan,610072;Longquanyi District Hospital of Traditional Chinese Medicial,Chengdu,Sichuan,610100;Chengdu University of TCM,Chengdu,Sichuan,610075)

机构地区：[1]成都中医药大学附属医院,四川成都610072 [2]龙泉驿区中医医院,四川成都610100 [3]成都中医药大学,四川成都610075

出　　处：《中医眼耳鼻喉杂志》2017年第4期203-205,211,共4页Journal of Chinese Ophthalmology and Otorhinolaryngology

摘　　要：目的观察补精益视片对慢性高眼压模型(elevatedintraocularpressure,EIOP)大鼠外侧膝状体中脑源性神经营养因子(brainderivedneurotrophicfactor,BDNF)表达的影响,探讨补精益视片视功能保护的作用机理。方法选取30只SD大鼠,随机分为对照组、给药组及模型组,每组各10只。采用烙闭巩膜上静脉方法制作慢性高眼压大鼠动物模型,每组连续灌胃8周后处死大鼠,观察补精益视片对EIOP大鼠眼压、外侧膝状体(lateralgeniculatenucleus,LGN)BDNF表达的影响。结果相比造模前,从造模即刻开始至造模后8周,给药组及模型组大鼠的眼压均有明显升高,差异具有统计学意义(均P<0.01);造模后8周与造模即刻相比,给药组眼压有降低趋势,差异有统计学意义(P<0.01),而模型组眼压无降低趋势;模型组BDNF总面积(55863.31±10264.399S/μm2)及积分光密度(15931.84±3403.742)较对照组(114106.08±17849.482S/μm2,29209.88±5725.992)及给药组(97567.06±22224.041S/μm2,29199.65±7865.087)明显偏低,差异具有统计学意义(均P<0.01),给药组BDNF总面积及积分光密度较对照组稍偏低,差异无统计学意义(P>0.05)。结论补精益视片能补精益视片能促进慢性EIOP模型大鼠LGN中BDNF的表达,从而发挥视功能保护作用。ObjectiveTo observe the effect of BuJingYiShi tablets on the expression of brain derived neurotrophic factor(BDNF)in lateral geniculate nucleus(LGN)in rat model of chronic elevated intraocular pressure(EIOP),and explore the mechanism of it initially.MethodsThe rats were randomly divided into3groups:control group,model group and treatment group,10rats in each group.The model of chronic EIOP was established by unilaterally cauterizing3episcleral vessels.After given BuJingYiShi tablets and normal saline for8weeks,killed the rats and taken out the brains.IOP,expression of brain derived neurotrophic factor in lateral geniculate nucleus(LGN)were observed.ResultsImmediately modeling and8weeks after modeling,IOP were highly significant different from before modeling(all P<0.01).there was a decreasing tendency in treatment group between8weeks after modeling and immediately modeling(P<0.01).While model group was not statistically significant(P>0.05).The total area(55863.31±10264.399S/μm2)and integrated optical density(15931.84±3403.742)of BDNF in model groups were lower than treatment group(114106.08±17849.482S/μm2,29209.88±5725.992)and control group(97567.06±22224.041S/μm2,29199.65±7865.087)(all P<0.01);Though total area and integrated optical density in treatment group were little lower than control group,there was no significant difference in statistics(P>0.05).ConclusionBuJingYiShi tablets can protect the visual function on the rat model of chronic EIOP by improving the expression of BDNF.

关 键 词：青光眼 补精益视片 视功能保护 大鼠慢性高眼压模型 脑源性神经营养因子 外侧膝状体 

分 类 号：R77[医药卫生—眼科]