天麻素对焦虑样胃高敏感FD模型大鼠胃敏感性和焦虑样行为的影响  

Effect of Gastrodin on Gastric Sensitivity and Anxiety-like Behavior in FD Rats With Anxiety-like Gastric Hypersensitivity

作　　者：景富春[1,2] 张军[1] 王进海[1] 冯诚[1] 杨宝德[3] 胡浩 孙晓明 郑建云[5] 尹晓然[1] 年媛媛[1] JING Fuchun;ZHANG Jun;WANG Jinhai;FENG Cheng;YANG Baode;HU Hao;SUN Xiaoming;ZHENG Jianyun;YIN Xiaoran;NIAN Yuanyuan(Department of Gastroenterology, the Second Affiliated Hospital of Xian Jiaotong University, Xian (710004);Department of Digestive Diseases, Baoji People’s Hospital Affiliated to Yanan University, Baoji, Shaanxi Province,Xian (710004);Department of Pharmacology, Health Science Center, Xian Jiaotong University, Xian(710004);Basic Medical Experiment Teaching Center, Health Science Center, Xian Jiaotong University, Xian(710004);Department of Pathology, the First Affiliated Hospital of Xian Medical University, Xian(710004))

机构地区：[1]西安交通大学第二附属医院消化内科,710004 [2]延安大学附属宝鸡市人民医院消化内科 [3]西安交通大学医学部药理学系 [4]西安交通大学医学部基础医学实验教学中心 [5]西安医学院第一附属医院病理科

出　　处：《胃肠病学》2017年第12期733-737,共5页Chinese Journal of Gastroenterology

摘　　要：背景:伴焦虑症状并以胃高敏感为主的功能性消化不良(FD)是临床治疗的难点之一,天麻素(Gas)可能具有调节胃敏感性和焦虑样行为的双重作用。目的:探讨Gas对焦虑样胃高敏感FD模型大鼠胃敏感性和焦虑样行为的影响。方法:将40只新生幼鼠随机分为对照组、模型组、丁螺环酮组、Gas低剂量组和Gas高剂量组。序贯采用母婴分离、急性胃刺激和束缚应激法制作焦虑样胃高敏感大鼠模型。第8周,对照组和模型组大鼠腹腔注射0.9%Na Cl溶液2.0 mL/kg,丁螺环酮组给予丁螺环酮3.125 mg/kg,Gas低、高剂量组分别给予62.5 mg/kg和125.0 mg/kg Gas,连续7 d。末次给药后行高架十字迷宫(EPM)试验、旷场试验、腹壁回撤反射(AWR)和肌电图(EMG)。结果:与对照组相比,EPM试验示模型组大鼠进入开放臂次数的比例、开放臂停留时间的比例均显著降低(P<0.01),旷场试验示虚拟中央格停留时间明显缩短(P<0.05)、穿行格数和直立次数明显减少(P<0.01);胃气囊扩张压力为40 mm Hg或以上时,AWR评分显著升高(P<0.05),EMG的ROC曲线下面积(AUC)明显增加(P<0.01)。而与模型组相比,高、低剂量Gas均可明显改善上述指标(P<0.05)。结论:Gas对焦虑样胃高敏感FD模型大鼠脑-胃轴调节中胃敏感性和焦虑样行为具有明显的影响。Functional dyspepsia(FD)with anxiety and gastric hypersensitivity is still one of the therapeutic difficulties in clinic.Gastrodin(Gas)may have dual effects of modulating gastric sensitivity and anxiety.Aims:To investigate the effect of Gas on gastric sensitivity and anxiety-like behavior in FD with anxiety-like gastric hypersensitivity in rats.Methods:Forty rats were randomly divided into control group,model group,buspirone group,low-dose Gas group and high-dose Gas group.Maternal separation,acute gastric irritation and restraint stress were sequentially performed to induce FD model with anxiety-like gastric hypersensitivity.At the8th week,rats in control group and model group were intraperitoneally injected with0.9%NaCl solution2.0mL/kg,rats in buspirone group were given buspirone3.125mg/kg,and rats in low-and high-dose Gas groups were given62.5,125.0mg/kg Gas,respectively.The course was7days.Then elevated plus maze(EPM),open field test,abdominal withdrawal reflex(AWR)and electromyography(EMG)were performed.Results:Compared with control group,EPM test showed that proportions of open arms entries and duration were significantly decreased(P<0.01);open field test showed that virtual central grids duration(P<0.05),number of virtual grids climbed and times of lifting were significantly decreased(P<0.01);when gastric balloon dilatation pressure was equal or greater than40mm Hg,AWR score,area under ROC curve(AUC)of EMG was significantly increased in model group(P<0.05).Compared with model group,above-mentioned indices in low-and high-dose Gas groups were significantly ameliorated(P<0.05).Conclusions:Gas could influence the gastric sensitivity and anxiety-like behavior of the brain-stomach axis regulated anxiety-like gastric hypersensitivity in FD rat model.

关 键 词：天麻素 焦虑 胃高敏感 消化不良 

分 类 号：R-332[医药卫生] R57