利用TCGA数据库分析miR-196b在结直肠癌患者中的临床表达特征并探索miR-196b的体外抗5-FU作用  

Analyzing clinical characteristic of miR-196b in human colorectal cancer using TCGA and effect of miR-196b on 5-FU resistance of CRC cell line

作　　者：车佳 黄兰兰[1] 娄琼 杨湘玲[1] 刘焕亮[1,2] CHE Jia;HUANG Lanlan;LOU Qiong;YANG Xiangling;LIU Huanliang(Guangdong Institute of Gastroenterology,The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China;Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China)

机构地区：[1]中山大学附属第六医院1广东省胃肠病学研究所,广东广州510655 [2]中山大学附属第六医院检验科,广东广州510655

出　　处：《中国病理生理杂志》2018年第1期152-157,共6页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.2015A030310096);广东省科技计划(No.2014B020212016)

摘　　要：目的:研究微小RNA(miR)-196b在结直肠癌患者中的表达及其临床意义,探讨过表达miR-196b的结直肠癌细胞对5-氟尿嘧啶(5-FU)的敏感性。方法:在癌症基因组图谱(The Cancer Genome Atlas,TCGA)公共数据库下载结直肠癌患者miRNA测序数据及其对应的临床病理资料,运用SPSS 17.0分析miR-196b在结直肠癌患者中的表达水平以及临床特征;采用瞬时转染的方法在人结直肠癌HCT116细胞中过表达miR-196b,并用MTS法分析过表达miR-196b的细胞对5-FU药物敏感性的变化。结果:miR-196b高表达与结直肠癌患者淋巴结转移及TNM分期相关(P<0.05),与年龄和性别等无相关性。淋巴结转移和远处转移是直肠癌患者生存的独立影响因子(P<0.05),miR-196b的表达水平与患者生存状况无关。过表达miR-196b的HCT116细胞在不同浓度的5-FU作用下,细胞存活率均明显高于对照组(P<0.05)。结论:miR-196b可能是结直肠癌患者肿瘤分期和淋巴结转移的分子标志物,并可降低结直肠癌细胞对5-FU的敏感性。AIM:To investigate the clinical characteristics of microRNA(miR)-196b in colorectal cancer(CRC)and to study its biological function in5-fluorouracil(5-FU)resistance.METHODS:miRNA sequence dataset and the corresponding clinical data of CRC patients were downloaded from The Cancer Genome Atlas(TCGA).Expression level and clinical characteristics of miR-196b in CRC patients were analyzed using SPSS17.0.CRC cell line overexpres-sing miR-196b was established using transient transfection method.MTS test was used to evaluate the effect of miR-196b overexpression on5-FU resistance.RESULTS:miR-196b expression was associated with lymph node metastasis and TNM stage(P<0.05),but not related with age and sex.Lymph node metastasis and distant metastasis were independent prognostic factors for rectal patients(P<0.05).The expression level of miR-196b was not associated with survival condition of rectal patients.The viability of the cells overexpressing miR-196b treated with different concentrations of5-FU was significantly higher than that in control group(P<0.05).CONCLUSION:miR-196b may be a potential biomarker of TNM stage and lymph node metastasis in CRC.miR-196b increases the5-FU resistance of CRC cells.

关 键 词：结直肠癌 癌症基因组图谱 微小RNA-196b 5-氟尿嘧啶 抗药性 

分 类 号：R735.3[医药卫生—肿瘤] R363[医药卫生—临床医学]