PUFA对LPS致ALI大鼠的氧化应激及TLR4、TNF-α的影响  

Effects of PUFA on oxidative stress and TLR4,TNF-alpha in ALI rats induced by LPS

作　　者：芦玲 白琳[1] 武猛[1] 丛沛然 郝鑫 吴玉荣[1] LU Lin;BAI Lin;WU Meng;CONG Peiran;HAO Jin;WU Yurong(Department ofIntensive Medicine,Hospital of Heilongjiang Province,Harbin,Heilongjiang 150036,China)

机构地区：[1]黑龙江省医院重症医学科,哈尔滨150036

出　　处：《国际检验医学杂志》2017年第24期3383-3385,共3页International Journal of Laboratory Medicine

基　　金：黑龙江省自然科学基金资助项目(QC2016127)

摘　　要：目的探究并分析ω-3多不饱和脂肪酸(PUFA)对内毒素脂多糖(LPS)致急性肺损伤(ALI)大鼠的氧化应激及Toll样受体-4(TLR4)、肿瘤坏死因子α(TNF-α)的影响。方法选取健康成年Wistar大鼠120只(清洁级,体质量180~240g),按照随机数字表法将其分为对照组和观察组,每组60只,对照组大鼠又随机分为未处理组、处理后6h组(LPS注射)、处理后24h组(LPS注射),每组20只。观察组大鼠又随机分为未处理组、处理后6h组(PUFA+LPS注射)、处理后24h组(PUFA+LPS注射),每组20只。观察2组大鼠处理前、处理后6h、处理后24h超氧化物歧化酶活性(SOD)、丙二醛(MDA)、细胞间黏附分子-1(ICAM-1)、TNF-α、TLR4相对表达量。结果观察组和对照组大鼠处理前后SOD水平比较差异有统计学意义(P<0.05);观察组和对照组大鼠处理前SOD水平比较差异无统计学意义(P>0.05);随着处理时间的推移,2组大鼠SOD水平均有所降低,但观察组大鼠降低程度低于对照组,差异有统计学意义(P<0.05);2组大鼠处理前后MDA水平比较差异有统计学意义(P<0.05);随着处理时间的推移,2组大鼠MDA水平均有所升高,但观察组大鼠升高程度明显低于对照组,差异有统计学意义(P<0.05);2组大鼠处理前后ICAM-1水平比较差异有统计学意义(P<0.05);随着处理时间的推移,2组大鼠ICAM-1、TNF-α水平有所升高,但观察组大鼠升高程度明显低于对照组,差异有统计学意义(P<0.05);观察组大鼠肺组织TLR4相对表达量低于对照组,差异有统计学意义(P<0.05)。结论 PUFA能有效降低LPS致ALI大鼠的氧化应激反应及炎症水平,可能有助于ALI的治疗。Objective To explore and analyze the effects ofω-3polyunsaturated fatty acid(PUFA)on oxidative stress and Toll-like receptor-4(TLR4)and tumor necrosis factor alpha(TNF-α)in rats with acute lung injury(ALI)induced by lipopolysaccharide(LPS).Methods120healthy adult Wistar rats(clean grade;weight,180-240g)were randomly divided into control group and observation group,60rats in each group,rats in the control group were randomly divided into untreated group,6h group(LPS injection),and24h group(LPS injection),20rats in each group.The rats in the observation group were randomly divided into untreated group,6h group(PUFA+LPS injection),and24h group(PUFA+LPS injection),20rats in each group.The relative expression of Superoxide Dismutase(SOD)before treatment,6h after treatment and24h after treatment,Malondialdehyde(MDA),intercellular cell adhesion molecule-1(ICAM-1),TNF-α,and TLR4in two groups were observed.ResultsThe difference of SOD levels before and after treatment in the two groups of rats was statistically significant(P<0.05),the difference of SOD levels before treatment in the two groups of rats was not statistically significant(P>0.05).As the processing time went on,the level of SOD in the two groups of rats decreased,but the decrease of the rats in the observation group was lower than that of the control group,and the difference was statistically significant(P<0.05),the difference of MDA level before and after treatment in two groups of rats was statistically significant(P<0.05).As the processing time went on,The level of MDA in the two groups of rats increased,but the increase of the rats in the observation group was significantly lower than that of the control group,and the difference was statistically significant(P<0.05).The difference of ICAM-1level before and after treatment in the two groups of rats was statistically significant(P<0.05).As the processing time went on,the levels of ICAM-1and TNF-alpha in the two groups increased,but the increase of the rats in the observation group was significantly lower

关 键 词：多不饱和脂肪酸 内毒素脂多糖 急性肺损伤 氧化应激 

分 类 号：R563.8[医药卫生—呼吸系统]