LSD1对非小细胞肺癌细胞增殖与迁移的影响  

Effect of LSD1 on proliferation and migration of non-small cell lung cancer cell

作　　者：洪娟[1] 王冬青[1] 殷瑞根[1] 赵天[1] 朱彦[1] HONG Juan;WANG Dong-qing;YING Rui-gen;ZHAO Tian;ZHU Yan(Department of Medical Imaging,Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001,China)

机构地区：[1]江苏大学附属医院影像科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2018年第1期34-38,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省社会发展项目(BE2015668)

摘　　要：目的:探讨赖氨酸特异性组蛋白去甲基化酶1(lysine-specific demethylase 1,LSD1)在非小细胞肺癌(NSCLC)中的表达及对细胞增殖与迁移的影响。方法:qRT-PCR检测52例NSCLC标本和对应癌旁组织中LSD1mRNA水平,分析其与NSCLC临床病理特征的关系。qRT-PCR检测NSCLC细胞A549与正常肺支气管上皮细胞16HBE中LSD1 mRNA表达;将A549细胞分为2组,分别转染LSD1干扰RNA(si-LSD1组),阴性对照RNA(对照组);MTT实验、克隆形成实验、流式细胞技术、Transwell迁移实验分别检测两组细胞增殖、凋亡、迁移能力的差异;qRT-PCR和蛋白质印迹法分别检测细胞上皮间质转化相关分子mRNA和蛋白水平。结果:与癌旁组织相比,NSCLC组织中LSD1 mRNA表达升高(t=2.861,P<0.05);LSD1表达量与NSCLC患者TNM分期及淋巴结转移相关(χ~2=4.062,6.047,P均<0.05),与患者年龄、性别、吸烟、肿瘤直径、病理分型无明显相关性(P>0.05)。A549细胞中LSD1 mRNA表达量明显高于16HBE细胞(t=3.569,P<0.05)。与对照组相比,si-LSD1组A549细胞增殖与迁移能力降低,细胞凋亡率显著增加(t=3.618,P<0.05),E-钙黏蛋白表达上调(t=5.607,P<0.05),波形蛋白表达下调(t=-6.628,P<0.05)。结论:LSD1在NSCLC组织及细胞中表达上调,可能通过促进上皮间质转化参与细胞增殖和迁移调控。To investigate the expression of lysine-specific demethylse1(LSD1)in non-small cell lung cancer(NSCLC)and its effect on cell proliferation and migration.Methods:The expression of LSD1mRNA in52pairs of NSCLC tissues and adjacent tissues were determined by qRT-PCR;and the relationship between LSD1expression and clinical features were analyzed.Expression of LSD1mRNA in NSCLC cell A549and normal lung bronchial epithelial cells16HBE were determined by qRT-PCR.A549cells were divided into2groups,transfected with LSD1interfering RNA(si-LSD1group)and negative control RNA(control group),respectively.Cell proliferation,apoptosis and migration in the two groups were detected by MTT assay,clone formation assay,flow cytometry,and Transwell migration test.Epithelial-mesenchymal transition related mRNA and protein expression levels were detected by qRT-PCR and Western blotting.Results:Compared with adjacent tissues,LSD1in NSCLC was significantly up-regulated(t=2.861,P<0.05).The expression of LSD1was correlated with TNM stage and lymph node metastasis in NSCLC patients(χ2=4.062,6.047,P<0.05).There was no significant correlation between LSD1expression and age,sex,smoking,tumor size and pathological type.Compared with the control group,si-LSD group showed lower proliferation and metastasis,higher rate of cell apoptosis(t=3.618,P<0.05),upregulated expression of E-cadherin(t=5.607,P<0.05)and downregulated expression of vimentin(t=-6.628,P<0.05).Conclusion:The expression of LSD1was up-regulated in NSCLC.LSD1might promote proliferation and metastasis by regulating EMT related gene.

关 键 词：赖氨酸特异性组蛋白去甲基化酶1 非小细胞肺癌 增殖 迁移 上皮间质转化 

分 类 号：R735.7[医药卫生—肿瘤]