三氧可通过减轻小鼠脑小胶质细胞激活预防抑郁症发生  

Ozone intraperitoneal injection prevents the depression by ameliorating microglia re-activation in depressed mice

作　　者：芦国芳 安建雄[2] 马钧 钱晓焱 王永[2] 杨小荣[1] LU Guofang;AN Jianxiong;MA Jun;QIAN Xiaoyan;WANG Yong;YANG Xiaorong(Key Laboratory for Cellular Physiology of Ministry ol Education, Department of Neurobiology,Shanxi Medical University, Taiyuan Shanxi 030001,China;Department of Anesthesiology,Pain Medicine and Critical Care Medicine, Aviation General Hospital of Chinese Medical University,Beijing Institute of Translational Medicine, Chinese Academy of Science, Beijing 100012,China)

机构地区：[1]山西医科大学基础医学院生理学系,教育部细胞生理学重点实验室,山西太原030001 [2]中国医科大学航空总医院暨中国科学院北京转化医学研究院麻醉、疼痛与重症医学中心,北京100012

出　　处：《转化医学杂志》2018年第1期20-26,共7页Translational Medicine Journal

基　　金：国家自然科学基金(81671076);国家青年科学基金(31000481);山西省自然科学基金(2011011040-2)

摘　　要：目的探究三氧对抑郁症模型小鼠行为学以及脑内海马、额叶和杏仁核小胶质细胞数量与炎症因子水平的影响。方法将40只小鼠随机分为4组,模型组、模型加三氧组、对照组和对照加三氧组,每组10只。对模型组和模型加三氧组小鼠进行连续7 d,每天1 h的束缚应激制备抑郁症小鼠模型,其中模型加三氧组和对照加三氧组采用三氧水(80μg/m L)腹腔注射。造模前3天给予三氧作为三氧预防性干预,造模成功后给予三氧作为三氧治疗性干预。利用悬尾实验、强迫游泳实验、新环境抑制进食实验和开放旷场实验检测各组小鼠行为学变化。小胶质细胞标记物IBA1免疫组化染色检测海马、额叶和杏仁核小胶质细胞数量的改变。逆转录聚合酶链式反应技术检测海马、额叶和杏仁核炎症因子白介素(interleukin,IL)-1β、IL-6和肿瘤坏死因子(tumer necrosis factor-α,TNF-α)的表达水平。结果三氧预防性干预中:三氧能改善小鼠抑郁行为,减少脑内额叶、海马区激活的小胶质细胞数量,降低炎症因子IL-1β、TNF-α的水平。三氧治疗性干预中:三氧未能改善小鼠抑郁行为,模型加三氧组与模型组额叶、海马、杏仁核区激活的小胶质细胞数量比较差异无统计学意义(P>0.05),三氧治疗性干预未能降低额叶、海马区炎症因子IL-1β、TNF-α的水平。结论三氧腹腔注射对预防小鼠抑郁症形成有一定的作用,但无治疗作用。Objective Explore the impact of ozone on depression model,including the behaviors,the number of microglia cell and inflammation cytokines(IL-1β,IL-6and TNF-α)in brain hippocampus,prefrontal cortex and amygdaloidal nucleus regions.Methods Forty mice were randomly divided into4groups:stress+saline(10),stress+ozone(10),controf+saline(10),controf+ozone(10).The model mice were set in a special centriluge tube for1hour per day,lasting fora week,ozone by intraperitoneal injection of controf+ozone and stress+ozone.Giving ozone3daysbelore depression model as a ozone previously intervention.Giving ozone alter depression model as aozone therapeutic intervention.Tail suspension test(TST),lorced swimming test(FST),noveltysuppressed leeding test(NSFT),open field test(OFT)were subjected to detect the behavior of eachgroup.Using the immunohistochemical technofogy to detect the number of microglia cell in brain hippocampus,prelrontal cortex and amygdaloidal nucleus regions.Using reverse transcription pofymerasechain reaction technofogy to detect the expression level of inflammatory cytokines IL-1β,IL-6andTNF-αlrom hippocampus,prelrontal cortex and amygdala region.Results Alter ozone previouslyintervention,ozone could improve depression behaviors in mice,reduce the number of activatedmicroglial cells in the prelrontal cortex and hippocampus,decrease the levels of inllammatory cytokines IL-iβ,TNF-α.Alter ozone intervention,ozone could not improve the depression behaviors in mice.There was no significant difference in the number of activated microglial cells in the prefrontal cortex,hippocampus and amygdala between stress+saline group and stress+ozone group(P>0.05),ozone treatment failed to reduce the levels of IL-iβand TNF-αin the prefrontal cortex and hippocampus.Conclusion Ozone intraperitoneal injection has some effects on preventing the formation of depression,but there is no therapeutic effects on depression.

关 键 词：三氧 抑郁 小胶质细胞 炎症因子 小鼠 

分 类 号：R749.4[医药卫生—神经病学与精神病学]