杜仲雄花化学成分的液相色谱-电喷雾三重四极杆飞行时间质谱分析  

Analysis of Chemical Constituents in Male Flowers of Eucommia ulmoides by Liquid Chromatography Coupled with Electrospray Ionization-Triple Quadrupole-Time of Flight-Tandem Mass Spectrometry(LC-ESI-Triple TOF-MS/MS)

作　　者：严颖[1] 赵慧[1] 邹立思[1] 刘训红[1] 柴川[1] 王胜男[1] 华愉教 YAN Ying;ZHAO Hui;ZOU Lisi;LIU Xunhong;CHAI Chuan;WANG Shengnan;HUA Yujiao(School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China)

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《食品科学》2018年第6期215-221,共7页Food Science

基　　金：江苏高校优势学科建设工程资助项目(YSXK-2014);江苏高校品牌专业建设工程资助项目(PPZY2015A070)

摘　　要：采用液相色谱-电喷雾三重四极杆飞行时间质谱法对杜仲雄花中化学成分进行分析。用反相C18色谱柱,以0.1%甲酸溶液(A)-乙腈(B)为流动相进行梯度洗脱;采用电喷雾离子源负离子扫描方式对样品进行分析。根据高分辨质谱提供的准分子离子和碎片离子的精确分子质量信息,结合标准品对照与参考相关文献数据,共鉴定出32种化学成分,包括2种木脂素类、9种环烯醚萜类、8种苯丙素类、12种黄酮类和1种酚苷类成分。上述结果可为探究杜仲雄花的药效物质基础及深度开发利用提供基础资料。The chemical constituents in male flowers of Eucommia ulmoides were analyzed by liquid chromatography coupled with electrospray ionization-triple quadrupole-time of flight-tandem mass spectrometry(LC-ESI-Triple TOF-MS/MS).The separation and analysis were carried out on a reversed-phase C18column with gradient elution using0.1%formic acid(A)-acetonitrile(B)under the negative ionization mode of ESI.According to the accurate mass of quasimolecular and product ions provided by Triple TOF-MS/MS,a total of32constituents were identified or tentatively presumed by comparing with reference standards and literature data,including2lignans,9iridoids,8penylpropanoids,12flavonoids and one phenolic glycoside.This study may provide basic information for further research on the pharmacodynamic material basis and intensive utilization of male flowers of Eucommia ulmoides.

关 键 词：杜仲雄花 液相色谱-电喷雾三重四极杆飞行时间质谱 化学成分 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]