双黄连口服液中多种有效成分测定  

Determination of Several Effective Components in Shuanghuanglian Oral Liquid

作　　者：张斌[1] Zhang Bin(Chemical Engineering College of Northwest Nationalities University, Gansu, 730030)

机构地区：[1]西北民族大学化工学院,甘肃730030

出　　处：《当代化工研究》2017年第11期137-139,共3页Modern Chemical Research

基　　金：西北民族大学中央高校基本科研业务费资金资助项目;项目编号(Y17009);项目名称:双黄连口服液中多种有效成分测定

摘　　要：目的:建立HPLC法同时测定双黄连口服液中绿原酸、栀子苷、丹皮酚和黄芩苷的含量。方法:采用安捷伦C18的色谱柱(4.6mm×150mm,5μm),流动相采用乙腈-0.6%磷酸的水溶液,梯度洗脱的流速为1.0m L·min^(-1),色谱柱温为30℃,检测波长分别为238nm(栀子苷),274nm(丹皮酚),280nm(黄芩苷),327nm(绿原酸)。结果:绿原酸、栀子苷、黄芩苷、丹皮酚进样量分别在0.005~0.173μg(r=0.9998)、0.026~1.231μg(r=0.9995)、0.041~1.682μg(r=0.9997)、0.006~0.249μg(r=0.9994)与峰面积呈良好的线性关系,平均回收率(n=6)分别为98.24%、99.10%、98.46%、98.65%,精密度RSD均小于1.0%,重复性RSD均小于1.0%,供试品溶液放置24h内稳定。含量测定的结果表明了不同生产企业之间各成分的含量不尽相同,部分生产企业不同生产批次之间也有较大的差异。结论:本法适用于同时测定双黄连口服液中绿原酸、栀子苷、黄芩苷和丹皮酚的含量。Objective:To establish a HPLC method for simultaneous determination of chlorogenic acid,geniposide,paeonol and baicalin in Shuanghuanglian oral liquid.Methods:The Agilent C18column(4.6mm×150mm,5μm),the mobile phase consisted of acetonitrile-0.6%phosphoric acid solution,the flow rate of gradient elution was1.0mL·min-1and the column temperature was30℃.The detection wavelength are respectively238nm(geniposide),274nm(paeonol),280nm(baicalin),327nm(chlorogenic acid)respectively.Results:The injection volumes of chlorogenic acid,geniposide,baicalin and paeonol were respectively0.005-0.1773μg(r=0.9998),0.026-1.231μg(r=0.9995)and0.041-1.682μg(r=0.9997),0.006~0.249μg(r=0.9994)and the peak area showed a good linear relationship.The average recovery(n=6)was98.24%,99.10%,98.46%and98.65%respectively.The RSDs of precision were less than1.0%and the repeatability RSDs were less than1.0%.The samples were stable within24h.Content determination results show that the content of different components of different manufacturers vary,some manufacturers have different production batches between the larger differences.Conclusion:This method is suitable for simultaneous determination of chlorogenic acid,geniposide,baicalin and paeonol in Shuanghuanglian Oral Liquid.

关 键 词：双黄连口服液 绿原酸 栀子苷 黄芩苷 丹皮酚 HPLC 

分 类 号：R[医药卫生]