大鼠奖励性条件反射实验中的影响因素  

The impact factors in reward conditioned reflex test of rats

作　　者：杨玉洁 王克柱 董黎明[3] 姜宁 范琳犀 张宏霞[5] 吕静薇[3] 陈善广[6] 刘新民[3] 王琼 YANG Yujie;WANG Kezhu;DONG Limi;JIANG Nin;FAN Linxi;ZHANG Hongxia;LYU Jingwei;CHEN Shanguan;LIU Xinmin;WANG Qiong(The Sino-Portugal TCM International Cooperation Center,Affiliated TCM Hospital,Southwest Medical University, Luzhou 646000,China;Department of Pharmacology,School of Pharmacy,Southwest Medical University,Luzhou 646000;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193;Guangdong East Sunshine Pharmaceutical Co.,Ltd,Dongguan 523808;Hunan University of Traditional Chinese Medicine,Changsha 410208;Astronaut Center of China,Beijing 100193)

机构地区：[1]西南医科大学附属中医医院中葡中医药国际合作中心,四川泸州646000 [2]西南医科大学药学院药理教研室,四川泸州646000 [3]中国医学科学院药用植物研究所,北京100193 [4]广东东阳光药业有限公司,广东东莞523808 [5]湖南中医药大学,长沙410208 [6]中国航天员科研训练中心,北京100193

出　　处：《中国比较医学杂志》2018年第3期8-14,共7页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划;政府间国际科技创新合作重点专项(编号:2016YFE0131800);中国医学科学院创新工程"人类疾病动物模型平台"(编号:2016-I2M-2-006)

摘　　要：目的研究不同颜色光刺激、刺激时长及昼/夜训练三种因素对大鼠奖励性条件反射实验的影响,为奖励性条件反射实验方法学提供数据依据。方法分为三批实验:实验一,选择8周龄Wistar大鼠分为3组,红光刺激组、黄光刺激组、蓝光刺激组;实验二,选择8周龄Wistar大鼠分为3组,灯亮持续10 s组、灯亮持续30 s组、灯亮持续60 s组;实验三,选择8周龄Wistar大鼠分为白天组(白天9:00训练)和夜晚组(晚上21:00训练)。所有实验动物均进行奖励性条件反射训练,统计正确鼻触次数、错误鼻触次数、鼻触正确率等指标。结果实验一:三组大鼠的正确鼻触次数、错误鼻触次数、运动路程、平均速度差异均无显著性。实验二:与灯亮10 s组相比,灯亮30 s组和灯亮60s组的正确鼻触次数显著升高(P<0.05);与灯亮10 s组比,灯亮30 s组在训练的第13天和第14天时的鼻触正确率显著增高(P<0.001)。实验三:两组大鼠的正确鼻触次数和鼻触正确率差异均无显著性。结论在奖励性条件反射中,红、黄、蓝光刺激所起到的奖励预测作用相似。同样的灯光刺激时,刺激时间的长短对其学习成绩有影响,适当延长灯光刺激时间有利于大鼠条件反射的形成。昼/夜训练对大鼠奖励性条件反射的获得无影响。Objective To study the effects of different color light stimulus,duration of stimulus and day/night training on the incentive conditioned reflex of rats,and to provide a data basis for the methodology of incentive conditional reflection experiments.Methods Three experiments were included in this study.During the experiment 1,a total of 30 male 8-week-old Wistar rats were divided into three groups:red light stimulation group,yellow light stimulation group and blue light stimulation group.During the experiment 2,a total of 32 male 8-week-old Wistar rats were divided into three groups:the group I(with a light stimulation duration of 10 s),the group II(with a light stimulation duration of 30 s),and the group III(with a light stimulation duration of 60 s).During the experiment 3,a total of 16 male 8-week-old Wistar rats were randomly divided into two groups,of which the first group was trained at 9:00 a.m.every day,and the second group was trained at 21:00 every day.All the laboratory animals were given reward conditioned reflex training,and indicators such as the number of correct nose pokes,the number of incorrect nose pokes,the ratio of correct nose pokes and so on were counted and calculated.Results During the experiment 1,there was no significant difference in the numbers of correct nose pokes and incorrect nose pokes,movement distances and average speeds of the rats among the three groups.During the experiment 2,compared with the group with a light duration of 10 s,the rats in the groups with a light duration of 30 s or 60 s had a higher number of correct nose pokes(P<0.05).Moreover,on the 13th and 14th days,the ratio of correct nose pokes of the group with a light duration of 30 s was significantly higher than that of the group with a light duration of 10 s(P<0.001).During the experiment 3,there were no significant differences in the number of correct nose pokes and the nose poke accuracy of the rats between the two groups.Conclusions In the reward conditioned reflex test,red,yellow and blue light stimuli have

关 键 词：光刺激 光刺激时长 昼/夜训练 奖励性条件反射 

分 类 号：R-33[医药卫生]