慢性胃肠黏膜损伤树鼩模型建立的探索  

Exploration of establishing a tree shrew model of chronic gastrointestinal mucosal injury

作　　者：叶尤松[1] 高家红[1] 赵汝 李哲丽[1] 王陈芸 马开利[1] 唐东红[1] YE Yousong;GAO Jiahong;ZHAO Ru;LI Zheli;WANG Chenyun;MA Kaili;TANG Donghong(Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China;School of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]中国医学科学院,北京协和医学院医学生物学研究所,昆明650118 [2]云南农业大学动物医学院,昆明650201

出　　处：《中国比较医学杂志》2018年第3期43-47,共5页Chinese Journal of Comparative Medicine

基　　金：中国医学科学院医学与健康科技创新工程重大协同创新项目(编号:2016-I2M-2-006);国家自然科学基金(编号:81571254,81570749)。

摘　　要：目的探索建立树鼩慢性胃肠黏膜损伤动物模型的可行性。方法将12只成年雄性树鼩随机分成3组,实验1、2组分别按2 mg/(kg·d)、1 mg/(kg·d)的1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)的剂量,对照组予同等体积的生理盐水,连续腹腔注射实验树鼩56 d,观察树鼩体重变化,检测脑脊液多巴胺含量,观察镜下胃肠形态和组织病理改变情况。结果模型组体重和脑脊液多巴胺含量明显下降,胃窦、胃体、十二指肠均有不同程度的病理变化,但2 mg/kg、1 mg/kg剂量组之间无明显区别,对照组未见明显改变。结论采用长期,小剂量腹腔注射MPTP方式,是一种可行的慢性胃肠黏膜损害树鼩模型的造模方法,最理想注射剂量为2 mg/(kg·d)。Objective To explore the feasibility of establishing a tree shrew model of chronic gastrointestinal mucosal injury.Methods A total of 12 adult male tree shrews were randomly divided into 3 groups.The experimental groups 1 and 2 were administered with intraperitoneal injection of 2 mg/(kg·d)and 1 mg/(kg·d)of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)once every day for 56 days,respectively.The control group was given the same volume of sterile saline at the corresponding time points.Changes in the body weight of the tree shrews were observed.The contents of dopamine in the cerebrospinal fluid were detected.Gastrointestinal morphology was observed by stereoscope and histopathological changes of the gastrointestinal mucosa were examined by HE staining.Results The body weight and the contents of dopamine in the cerebrospinal fluid of the tree shrews in the model group were significantly decreased(P<0.05 for both).Pathological changes to some extent of the gastric antrum,the gastric body and the duodenum were observed,without obvious differences between the 2 mg/kg group and the 1 mg/kg group.No obvious changes were found in the control group.Conclusions Long-term intraperitoneal injection with a low dose of MPTP is a feasible method for the establishment of a tree shrew model of chronic gastrointestinal mucosal injury.The optimal dose is 2 mg/(kg·d)every day for 56 days.

关 键 词：1-甲基-4-苯基-1 2 3 6-四氢吡啶 慢性胃肠黏膜损伤 树鼩 

分 类 号：R-33[医药卫生]