Brown-Norway大鼠咳嗽变异性哮喘模型的建立  

Establishment of a Brown-Norway rat model of cough variant asthma

作　　者：楚慧伦 孔德明[1] 丁子桐 高琴琴[1] 续艳[1] 阎玥[2] 李友林[2] 孙文燕[1] CHU Huilun;KONG Deming;DING Zitong;GAO Qinqin;XU Yan;YAN Yue;LI Youlin;SUN Wenyan(School of Chinese Materia Medicine,Beijing University of Chinese Medicine,Beijing 100102,China;Key Institute of Pneumopathy Chronic Cough and Dyspnea,State Administration of Traditional Chinese Medicine, China-Japan Friendship Hospital,Beijing 100102)

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中日友好医院国家中医药管理局肺病慢性咳嗽重点研究室,北京100102

出　　处：《中国比较医学杂志》2018年第3期63-66,共4页Chinese Journal of Comparative Medicine

基　　金：北京市科委十病十药项目(编号:Z151100003815025)

摘　　要：目的通过卵蛋白(OVA)致敏Brown-Norway(BN)大鼠,建立一种简单易行的咳嗽变异性哮喘动物模型。方法 36只BN大鼠分为三组:正常对照组、模型对照组、给药组。第1日大鼠腹腔内注射2 mg卵蛋白和100 mg Al(OH)3凝胶,3周后再次腹腔注射0.01 mg OVA及100 mg Al(OH)3凝胶,正常对照组大鼠腹腔注射等量生理盐水。3周后模型对照组和给药组用1%OVA雾化,正常对照组用生理盐水雾化,隔日一次,共7次。雾化当天给药组开始灌胃给药,其余两组给等量生理盐水,每天一次,共14次。最后一次给药24 h后,进行辣椒素引咳、肺功能实验(激发实验)。结果与正常对照组比较,模型对照组咳嗽次数显著增多(P<0.01),吸气总气道阻力(RL)显著上升(P<0.05),动态顺应性(Cdyn)显著下降(P<0.05);与模型对照组比较,给药组的咳嗽次数显著减少(P<0.05),RL显著下降(P<0.05),Cdyn显著上升(P<0.05)。结论此种动物模型与咳嗽变异性哮喘多种临床特征相似,操作简便易行,故可作为咳嗽变异性哮喘的动物模型。Objective To establish a simple animal model of cough variant asthma(CVA)through sensitizing Brown-Norway(BN)rats with ovalbumin(OVA).Methods A total of 36 BN rats were randomly divided into three groups,including the normal control group,the model control group and the montelukast group.BN rats in the model group and the montelukast group were intraperitoneally administered with 2.0 mg of OVA and 100 mg of Al(OH)3,and the same volume of sterile saline was given to the normal group by intraperitoneal injection.Boosting was carried out by intraperitoneal administration with 0.01 mg of OVA and 100 mg of Al(OH)3 3 weeks later,and the rats in the normal group were injected with the same dose of physiological saline.Three weeks later,the actively sensitized BN rats were challenged with aerosolized OVA for 7 times on alternative days,and the rats in the normal group were treated with sterile saline instead of OVA.At the same time,the montelukast group was given 1.3 mg/kg of montelukast 30 minutes before atomization by intragastric administration once a day for 2 weeks,and the normal group and the model group were given the same volume of water.The tests of cough sensitivity to capsaicin and bronchial responsiveness were performed 24 h after the last administration.Results Compared with the normal group,the times of coughing(P<0.01)and the lung resistance(R L)(P<0.05)in the model group were significantly increased,while the lung compliance(C dyn)was significantly decreased(P<0.05).There was a significant difference(P<0.05)in the times of coughing caused by capsaicin between the model group and the montelukast group.Compared with the model group,R L in the montelukast group was decreased significantly(P<0.05),and C dyn was increased significantly(P<0.05).Conclusions This rat model of CVA is similar to a variety of clinical features of CVA and is easy to operate.Thus it can be used as an effective animal model of CVA.

关 键 词：咳嗽变异性哮喘 动物模型 BN大鼠 卵蛋白 肺功能 

分 类 号：R-33[医药卫生]